Author, as appears in the article.: Guerrini L; Alvarez-Puebla RA

Department: Química Física i Inorgànica

e-ISSN: 2079-4991

URV's Author/s: Alvarez Puebla, Ramon Angel / Guerrini, Luca

Keywords: Triplex Surface-enhanced raman spectroscopy Plasmonic nanoparticles Dna

Abstract: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.

Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: ramon.alvarez@urv.cat luca.guerrini@urv.cat

Author identifier: 0000-0003-4770-5756 0000-0002-2925-1562

Record's date: 2023-02-19

Journal volume: 11

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2079-4991/11/2/326

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nanomaterials. 11 (2): 1-9

APA: Guerrini L; Alvarez-Puebla RA (2021). Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy. Nanomaterials, 11(2), 1-9. DOI: 10.3390/nano11020326

Article's DOI: 10.3390/nano11020326

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications