Author, as appears in the article.: Marinho S; Illanes M; Ávila-Román J; Motilva V; Talero E

Department: Bioquímica i Biotecnologia

e-ISSN: 2218-273X

URV's Author/s: Ávila Román, Francisco Javier

Keywords: Ulcerative colitis Rosmarinic acid Nrf2 Nanovesicles Inflammasome

Abstract: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Ulcerative colitis (UC), one of the two main types of inflammatory bowel disease, has no effective treatment. Rosmarinic acid (RA) is a polyphenol that, when administered orally, is metabolised in the small intestine, compromising its beneficial effects. We used chitosan/nutriose-coated niosomes loaded with RA to protect RA from gastric degradation and target the colon and evaluated their effect on acute colitis induced by 4% dextran sodium sulphate (DSS) for seven days in mice. RA-loaded nanovesicles (5, 10 and 20 mg/kg) or free RA (20 mg/kg) were orally administered from three days prior to colitis induction and during days 1, 3, 5 and 7 of DSS administration. RA-loaded nanovesicles improved body weight loss and disease activity index as well as increased mucus production and decreased myeloperoxidase activity and TNF-α production. Moreover, RA-loaded nanovesicles downregulated protein expression of inflammasome components such as NLR family pyrin domain-containing 3 (NLRP3), adaptor protein (ASC) and caspase-1, and the consequent reduction of IL-1β levels. Furthermore, nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein expression increased after the RA-loaded nanovesicles treatment However, these mechanistic changes were not detected with the RA-free treatment. Our findings suggest that the use of chitosan/nutriose-coated niosomes to increase RA local bioavailability could be a promising nutraceutical strategy for oral colon-targeted UC therapy.

Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: franciscojavier.avila@urv.cat

Author identifier: 0000-0001-9766-8178

Record's date: 2023-02-19

Journal volume: 11

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-273X/11/2/162

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomolecules. 11 (2): 1-17

APA: Marinho S; Illanes M; Ávila-Román J; Motilva V; Talero E (2021). Anti-inflammatory effects of rosmarinic acid-loaded nanovesicles in acute colitis through modulation of NLRP3 inflammasome. Biomolecules, 11(2), 1-17. DOI: 10.3390/biom11020162

Article's DOI: 10.3390/biom11020162

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications