Articles producció científica> Medicina i Cirurgia

Liposcale: A novel advanced lipoprotein test based on 2D diffusion-ordered 1H NMR spectroscopy

  • Identification data

    Identifier: PC:1166
    Authors:
    Correig, X.Masana, L.Yanes, O.Ribalta, J.Rock, E.Plana, N.Vinaixa, M.Heras, M.Rodríguez, M.A.Amigó, N.Mallol, R.
    Abstract:
    Determination of lipoprotein particle size and number using advanced lipoprotein tests (ALTs) is of particular importance to improve cardiovascular risk prediction. Here we present the Liposcale test, a novel ALT based on 2D diffusion-ordered 1H NMR spectroscopy. Our method uses diffusion coefficients to provide a direct measure of the mean particle sizes and numbers. Using 177 plasma samples from healthy individuals and the concentration of ApoB and ApoA from isolated lipoprotein fractions, our test showed a stronger correlation between the NMR-derived lipoprotein particle numbers and apolipoprotein concentrations than the LipoProfile ® test commercialized by Liposcience. We also converted LDL particle numbers to ApoB equivalents (milligrams per deciliter) and our test yielded similar values of LDL-ApoB to the LipoProfile ® test (absolute mean bias of 8.5 and 7.4 mg/dl, respectively). In addition, our HDL particle number values were more concordant with the calibrated values determined recently using ion mobility. Finally, principal component analysis distinguished type 2 diabetic patients with and without atherogenic dyslipidemia (AD) on a second cohort of 307 subjects characterized using the Liposcale test (area under the curve = 0.88) and showed concordant relationships between variables explaining AD. Altogether, our method provides reproducible and reliable characterization of lipoprotein particles and it is applicable to pathological states such as AD. Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.
  • Others:

    Author, as appears in the article.: Correig, X. Masana, L. Yanes, O. Ribalta, J. Rock, E. Plana, N. Vinaixa, M. Heras, M. Rodríguez, M.A. Amigó, N. Mallol, R.
    Department: Medicina i Cirurgia Enginyeria Electrònica
    URV's Author/s: CORREIG BLANCHAR, FRANCESC XAVIER MASANA MARÍN, LUIS YANES TORRADO, ÓSCAR RIBALTA VIVES, JOSEP Rock, E. PLANA GIL, NÚRIA VINAIXA CREVILLENT, MARIA HERAS IBAÑEZ, MERCEDES Rodríguez, M.A. Amigó, N. MALLOL PARERA, ROGER
    Keywords: Cardiovascular risk
    Abstract: Determination of lipoprotein particle size and number using advanced lipoprotein tests (ALTs) is of particular importance to improve cardiovascular risk prediction. Here we present the Liposcale test, a novel ALT based on 2D diffusion-ordered 1H NMR spectroscopy. Our method uses diffusion coefficients to provide a direct measure of the mean particle sizes and numbers. Using 177 plasma samples from healthy individuals and the concentration of ApoB and ApoA from isolated lipoprotein fractions, our test showed a stronger correlation between the NMR-derived lipoprotein particle numbers and apolipoprotein concentrations than the LipoProfile ® test commercialized by Liposcience. We also converted LDL particle numbers to ApoB equivalents (milligrams per deciliter) and our test yielded similar values of LDL-ApoB to the LipoProfile ® test (absolute mean bias of 8.5 and 7.4 mg/dl, respectively). In addition, our HDL particle number values were more concordant with the calibrated values determined recently using ion mobility. Finally, principal component analysis distinguished type 2 diabetic patients with and without atherogenic dyslipidemia (AD) on a second cohort of 307 subjects characterized using the Liposcale test (area under the curve = 0.88) and showed concordant relationships between variables explaining AD. Altogether, our method provides reproducible and reliable characterization of lipoprotein particles and it is applicable to pathological states such as AD. Copyright © 2015 by the American Society for Biochemistry and Molecular Biology, Inc.
    Research group: Signal Processing for Omic Sciences Yanes Lab Unitat de Recerca de Lípids i Arteriosclerosi
    Thematic Areas: Biochemistry and technology Bioquímica y tecnología Bioquímica i biotecnologia
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0022-2275
    Author identifier: 0000-0002-6902-3054 0000-0002-0789-4954 0000-0003-3695-7157 0000-0002-8879-4719 n/a n/a 0000-0001-9804-0171 n/a n/a n/a n/a
    Record's date: 2015-04-28
    Last page: 746
    Journal volume: 56
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: http://www.jlr.org/content/suppl/2015/01/07/jlr.D050120.DC1.html
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1194/jlr.D050120
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2015
    First page: 737
    Publication Type: Article Artículo Article
  • Keywords:

    Lipoproteïnes de baixa densitat
    Sistema cardiovascular -- Malalties -- Factors de risc
    Cardiovascular risk
    Biochemistry and technology
    Bioquímica y tecnología
    Bioquímica i biotecnologia
    0022-2275
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