Author, as appears in the article.: Svobodova, Marketa; Skouridou, Vasso; Jauset-Rubio, Miriam; Vieitez, Irene; Fernandez-Villar, Alberto; Cabrera Alvargonzalez, Jorge Julio; Poveda, Eva; Benavent Bofill, Clara; Sans, Teresa; Bashammakh, Abdulaziz; Alyoubi, Abdulrahman O.; O'Sullivan, Ciara K.;
Department: Enginyeria Química
URV's Author/s: Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
Keywords: Sensitive detection Selection Receptor-binding domain In-vitro Evolution Enzyme Discovery Coronavirus sensitive detection selection in-vitro evolution enzyme discovery coronavirus
Abstract: The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) emerged at the end of 2019, resulting in the ongoing COVID-19 pandemic. The high transmissibility of the virus and the substantial number of asymptomatic individuals have led to an exponential rise in infections worldwide, urgently requiring global containment strategies. Reverse transcription-polymerase chain reaction is the gold standard for the detection of SARS-CoV-2 infections. Antigen tests, targeting the spike (S) or nucleocapsid (N) viral proteins, are considered as complementary tools. Despite their shortcomings in terms of sensitivity and specificity, antigen tests could be deployed for the detection of potentially contagious individuals with high viral loads. In this work, we sought to develop a sandwich aptamer-based assay for the detection of the S protein of SARS-CoV-2. A detailed study on the binding properties of aptamers to the receptor-binding domain of the S protein in search of aptamer pairs forming a sandwich is presented. Screening of aptamer pairs and optimization of assay conditions led to the development of a laboratory-based sandwich assay able to detect 21 ng/mL (270 pM) of the protein with negligible cross-reactivity with the other known human coronaviruses. The detection of 375 pg of the protein in viral transport medium demonstrates the compatibility of the assay with clinical specimens. Finally, successful detection of the S antigen in nasopharyngeal swab samples collected from suspected patients further establishes the suitability of the assay for screening purposes as a complementary tool to assist in the control of the pandemic.
Thematic Areas: Química Interdisciplinar General chemistry General chemical engineering Engenharias ii Ciências agrárias i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemical engineering (miscellaneous) Chemical engineering (all)
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vasoula.skouridou@urv.cat miriam.jauset@urv.cat
Author identifier: 0000-0002-9712-5429 0000-0002-9943-6132
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://pubs.acs.org/doi/10.1021/acsomega.1c05521
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Acs Omega. 6 (51): 35657-35666
APA: Svobodova, Marketa; Skouridou, Vasso; Jauset-Rubio, Miriam; Vieitez, Irene; Fernandez-Villar, Alberto; Cabrera Alvargonzalez, Jorge Julio; Poveda, Eva (2021). Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen. Acs Omega, 6(51), 35657-35666. DOI: 10.1021/acsomega.1c05521
Article's DOI: 10.1021/acsomega.1c05521
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV