Avaluació de la toxicitat reproductiva de rates mascle a través d'exposició materna al dienestrol

Alfageme Abelló, Oscar

Belongs to TFG:SerieGeneralBIOT collection

TITLE:

Avaluació de la toxicitat reproductiva de rates mascle a través d'exposició materna al dienestrol - TFG:1643

Handle:

https://hdl.handle.net/20.500.11797/TFG1643

Student:	Alfageme Abelló, Oscar
Language:	cat
Title in original language:	Avaluació de la toxicitat reproductiva de rates mascle a través d'exposició materna al dienestrol
Title in different languages:	Assessment of reproductive toxicity of male rats through maternal exposure to dienestrol
Keywords:	dienestrol, endocrine disruptor, reproductive toxicity
Subject:	Bioquímica i biotecnologia
Abstract:	Dienestrol is an abortive compound that was used to treat menopausal symptoms and as an anabolic in cattle. It is also a diethylstilbestrol metabolite, which is a stilbene derivative with an estrogenic activity comparable to that of the natural estrogen, estradiol-17β. Dienestrol has been classified as an endocrine disruptor, altering the functions of the organism and causing possible harmful effects on offspring. At present, traces of this compound have been found in dairy foods and in the environment. The objective of this study is to determine if dienestrol affects men’s reproduction due to its estrogen agonistic activity. Female Sprague-Dawley rats were treated daily with gavage from gestational day 6 until postnatal day 21. Pups of the treated rats were sacrificed at 90 days and were divided into different groups according to the administered dose (1.5, 3 and 6.25 kg/body weight/day). Sperm and spermatid count, sperm motility, viability and morphology were examined. Histopathology of the testicle and epididymis was evaluated, and serum biochemical parameters were analyzed. The results indicate that there is a significant decrease in the count, viability and mobility of sperm after maternal exposure to dienestrol. Moderate histopathological affectations were observed in the seminiferous tubules in the testicle sections, and apoptosis of the epididymal duct epithelium was also seen. In addition, there is a significant decrease in the levels of total proteins and in the activity of alkaline phosphatase and alanine transaminase enzymes, and a significant increase in glucose and HDL. These results indicate that exposure to dienestrol in pregnant rats has toxic effects on reproductive capacity in the young male offspring.
Project director:	Gómez Arnaiz, Mercedes
Department:	Bioquímica i Biotecnologia
Education area(s):	Biotecnologia
Entity:	Universitat Rovira i Virgili (URV)
TFG credits:	9
Creation date in repository:	2017-10-29
Work's public defense date:	2017-06-28
Academic year:	2017-2018
Confidenciality:	No
Subject areas:	Biochemistry and biotechnology
Access rights:	info:eu-repo/semantics/openAccess

Search your record at:

Available files
File	Description	Format
Memòria	Memory	application/pdf

All objects of this collection

Type:	info:eu-repo/semantics/bachelorThesis
Contributor:	Gómez Arnaiz, Mercedes
Títol:	Avaluació de la toxicitat reproductiva de rates mascle a través d'exposició materna al dienestrol
Language:	cat
Subject:	Bioquímica y biotecnología Biochemistry and biotechnology Bioquímica i biotecnologia Bioquímica i biotecnologia
Creator:	Alfageme Abelló, Oscar
Rights:	info:eu-repo/semantics/openAccess
Date:	2017-06-28

Information