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TITLE:
Experimental Approaches for Detecting Long Noncoding RNAs in Malignant Pleural Effusion - TFG:6581
Handle: https://hdl.handle.net/20.500.11797/TFG6581

Student:Serrano Ribes, Núria
Language:en
Title in original language:Experimental Approaches for Detecting Long Noncoding RNAs in Malignant Pleural Effusion
Keywords:Malignant pleural effusion, biomarker, lncRNA
Subject:Embassament pleural
Abstract:This study focuses on establishing the optimal experimental conditions for detecting long noncoding RNAs (lncRNAs) which are tumour-derived products present in pleural fluid (PF). A qPCR assay was conducted to evaluate the impact of different initial sample volumes, the inclusion of an RNA spike-in (cel-miR-39) during the extraction process and the implementation of a preamplification step. The results suggest that the optimal conditions for lncRNA extraction from PF involve an initial volume of 200μL, the addition of cel-miR-39 and carrying out of a preamplification procedure. Furthermore, ACTB and GAPDH were validated as reference genes for data normalisation in PF samples.
Project director:Torrell Galceran, Helena
Department:Bioquímica i Biotecnologia
Education area(s):Bioquímica i Biologia Molecular
Entity:Universitat Rovira i Virgili (URV)
Creation date in repository:2023-12-18
Work's public defense date:2023-06-21
Academic year:2022-2023
Confidenciality:No
Subject areas:Biochemistry and biotechnology
Access rights:info:eu-repo/semantics/openAccess
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