Repositori institucional URV
| URV's Author/s: | BASAURE GARCÍA, PÍA ISABEL / Cabré Bargalló, Maria / Colomina Fosch, Maria Teresa / Domingo Roig, José Luis / Guardia Escoté, Laia |
| Author, as appears in the article.: | Basaure, Pia; Guardia-Escote, Laia; Cabre, Maria; Peris-Sampedro, Fiona; Sanchez-Santed, Fernando; Domingo, Jose L; Teresa Colomina, Maria |
| Author's mail: | maria.cabre@urv.cat joseluis.domingo@urv.cat mariateresa.colomina@urv.cat |
| Author identifier: | 0000-0003-4124-8603 0000-0001-6647-9470 0000-0002-5619-4874 |
| Journal publication year: | 2019 |
| Publication Type: | Journal Publications |
| ISSN: | 03405761 |
| APA: | Basaure, Pia; Guardia-Escote, Laia; Cabre, Maria; Peris-Sampedro, Fiona; Sanchez-Santed, Fernando; Domingo, Jose L; Teresa Colomina, Maria (2019). Learning, memory and the expression of cholinergic components in mice are modulated by the pesticide chlorpyrifos depending upon age at exposure and apolipoprotein E (APOE) genotype. Archives Of Toxicology, 93(3), 693-707. DOI: 10.1007/s00204-019-02387-9 |
| Paper original source: | Archives Of Toxicology. 93 (3): 693-707 |
| Abstract: | © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Polymorphisms of the apolipoprotein E (APOE) gene differentially affect neurobiological functions and cognitive performance and confer different vulnerabilities to subclinical exposures to chlorpyrifos (CPF), a pesticide used worldwide. The data reported on this topic suggest a complex interaction between cholinergic signaling and the APOE genotype. To gain greater functional insight into this interaction, we evaluated spatial learning and memory and hippocampal cholinergic expression in young apoE3 and apoE4 transgenic mice exposed to CPF. Male and female mice were exposed to CPF at 0 or 1 mg/kg on postnatal days 10–15 and then, exposed to CPF at 0 or 2 mg/kg for 60 days at 5 months of age. At 6 months of age, mice were tested for spatial skills in a Barnes maze. At the end of the task, animals were killed and gene expression of cholinergic components was assessed in the hippocampus. Our results show that apoE4 female mice performed worse in the spatial task, while postnatal CPF impaired escape strategies and spatial memory in apoE3 mice. In turn, CPF in adulthood improved spatial abilities in apoE4 female mice. Regarding gene expression, choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) expression were increased in apoE4 mice. Postnatal exposure to CPF increased ChAT mRNA levels in apoE4 mice, whereas adult exposure to CPF induced changes in acetylcholinesterase-S, α7- and α4-subunit nicotinic receptor expression in apoE4 females. The current findings provide new insights into APOE-dependent cholinergic signaling, which directly affects the response to CPF cholinergic insult, especially in APOE4 subjects. |
| Article's DOI: | 10.1007/s00204-019-02387-9 |
| Link to the original source: | https://link.springer.com/article/10.1007/s00204-019-02387-9 |
| Paper version: | info:eu-repo/semantics/acceptedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Psicologia Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Toxicology Saúde coletiva Química Psicología Odontología Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Health, toxicology and mutagenesis General medicine Farmacia Engenharias iii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade |
| Keywords: | Transgenic mice Targeted replacement Spatial memory Sex differences Receptors, nicotinic Pp swedish mice Postnatal exposure Pesticide Oxidative stress Nicotinic acetylcholine-receptors Nicotinic acetylcholine receptor alpha4 subunit Mice, transgenic Mice Memory Male Learning and memory Learning Insecticides Hippocampus Genotype Female Epsilon-4 allele Dietary exposure Cholinergic system Cholinergic agents Chlorpyrifos Behavioral consequences Apolipoproteins e Apolipoprotein e4 Apolipoprotein e3 Apolipoprotein e Animals Alzheimers-disease Age factors Acetylcholinesterase Acetylcholine pesticide memory learning cholinergic system apolipoprotein e |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2025-02-18 |
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