Repositori institucional URV
| URV's Author/s: | Figueras Salvat, María Josefa |
| Author, as appears in the article.: | Figueras, Maria José; Levican, Arturo; Collado, Luis; |
| Author's mail: | mariajose.figueras@urv.cat |
| Author identifier: | 0000-0002-2268-8980 |
| Journal publication year: | 2012 |
| Publication Type: | Journal Publications |
| APA: | Figueras, Maria José; Levican, Arturo; Collado, Luis; (2012). Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp.. Bmc Microbiology, 12(292), 292-. DOI: 10.1186/1471-2180-12-292 |
| Papper original source: | Bmc Microbiology. 12 (292): 292- |
| Abstract: | Background: Arcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease (MseI). The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter.
Results: Digestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and/or BfaI generated species-specific RFLP patterns.
Conclusions: 16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses. |
| Article's DOI: | 10.1186/1471-2180-12-292 |
| Link to the original source: | https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-12-292 |
| Papper version: | info:eu-repo/semantics/publishedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Ciències Mèdiques Bàsiques |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física |
| Keywords: | Taxonomy Sp nov. Polyacrylamide Pcr assay Multiplex Identification Helicobacter Gene Fragment-length-polymorphism Campylobacter Butzleri Arcobacter Agarose Aeromonas 16s rrna-rflp 16s rrna gene mutations |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2023-02-22 |
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