Repositori institucional URV
| URV's Author/s: | Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / GONZALEZ QUILEN, CARLOS ALBERTO / Pinent Armengol, Montserrat / Terra Barbadora, Ximena |
| Author, as appears in the article.: | González-Quilen C; Gil-Cardoso K; Ginés I; Beltrán-Debón R; Pinent M; Ardévol A; Terra X; Blay M |
| Author's mail: | anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat raul.beltran@urv.cat |
| Author identifier: | 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0001-9691-1906 |
| Journal publication year: | 2019 |
| Publication Type: | Journal Publications |
| ISSN: | 20726643 |
| e-ISSN: | 2072-6643 |
| APA: | González-Quilen C; Gil-Cardoso K; Ginés I; Beltrán-Debón R; Pinent M; Ardévol A; Terra X; Blay M (2019). Grape-seed proanthocyanidins are able to reverse intestinal dysfunction and metabolic endotoxemia induced by a cafeteria diet in wistar rats. Nutrients, 11(5), -. DOI: 10.3390/nu11050979 |
| Papper original source: | Nutrients. 11 (5): |
| Abstract: | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. We evaluated the effectiveness of pharmacological doses of grape-seed proanthocyanidin extract (GSPE) in reversing intestinal barrier alterations and local inflammation in female Wistar rats fed a long-term obesogenic diet. Animals were fed a 17-week cafeteria diet (CAF diet), supplemented with daily GSPE doses (100 or 500 mg kg-1 body weight) during the final two weeks. CAF diet enhanced the intestinal permeation of an orally administered marker (ovalbumin, OVA) and increased the plasma levels of tumor necrosis factor-α (TNF-α) and lipopolysaccharides (LPS) in 2-3-fold. Ex vivo Ussing chamber assays showed a 55-70% reduction in transepithelial electrical resistance (TEER) and increased the TNF-α secretions in both small and large intestinal sections with a 25-fold increment in the ileum. Ileal tissues also presented a 4-fold increase of myeloperoxidase (MPO) activity. Both GSPE-treatments were able to restitute TEER values in the ileum and colon and to reduce plasma LPS to basal levels without a dose-dependent effect. However, effects on the OVA permeation and TNF-α secretion were dose and section-specific. GSPE also reduced ileal MPO activity and upregulated claudin 1 gene expression. This study provides evidence of the efficacy of GSPE-supplementation ameliorating diet-induced intestinal dysfunction and metabolic endotoxemia when administered at the end of a long-term obesogenic diet. |
| Article's DOI: | 10.3390/nu11050979 |
| Link to the original source: | https://www.mdpi.com/2072-6643/11/5/979 |
| Papper version: | info:eu-repo/semantics/publishedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Bioquímica i Biotecnologia |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología |
| Keywords: | Vitis Ussing chamber Transepithelial electrical resistance Tight junction Supplementation Seeds Rich Rats, wistar Rats Proanthocyanidins Obesity Liver Intestinal diseases Insulin-resistance Inflammation High-fat diet Gut microbiota Gut Flavan-3-ol Female Extract Endotoxemia Dose-response relationship, drug Diet Barrier function Animals ussing chamber tight junction obesity inflammation gut flavan-3-ol |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2024-07-27 |
| Journal volume: | 11 |
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