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TITLE:
Microscopic imaging of human bloodstains: testing the potential of a confocal laser scanning microscope as an alternative to SEMs - imarina:6122648

URV's Author/s:Hortolà i Gómez, Policarp
Author, as appears in the article.:Hortolà P
Author's mail:policarp.hortola@urv.cat
Author identifier:0000-0003-2867-6531
Journal publication year:2020
Publication Type:Journal Publications
APA:Hortolà P (2020). Microscopic imaging of human bloodstains: testing the potential of a confocal laser scanning microscope as an alternative to SEMs. Micron, 130(UNSP 102821), 102821-. DOI: 10.1016/j.micron.2019.102821
Papper original source:Micron. 130 (UNSP 102821): 102821-
Abstract:© 2019 Elsevier Ltd The forensic interest on human bloodstains derives from their relation to crime investigation, whereas an archaeological and ethnographic concern arises from their occurrence because of warfare and ritual. The development of digital reflected light microscopes provided an opportunity to use ligh microscopy to study surface topographies in a more accurate way than previously. However, this enhancement has been focused on increasing magnification rather than resolution. An advanced type of light microscope is the confocal laser scanning microscope (CLSM). Its potential as an alternative to scanning electron microscopes (SEMs) for imaging human bloodstains was tested. A fragment of stone (brown chert) was smeared with human peripheral blood, air-dried, and stored indoors. After nearly two years, the sample was examined and imaged using an Olympus LEXT OLS4000 CLSM. The surface detail of CLSM images appeared to be comparatively lower than that of SEM micrographs of coated bloodstains taken at high-vacuum mode and high accelerating voltage, similar to that of SEM micrographs of uncoated bloodstains taken at low-vacuum mode and high accelerating voltage, and similar to or even higher than that of SEM micrographs of uncoated bloodstains taken at high-vacuum mode and low accelerating voltage. These results suggest that a CLSM is a practical alternative to SEMs for imaging human bloodstains when a very-high level of surface detail is not required.
Article's DOI:10.1016/j.micron.2019.102821
Link to the original source:https://www.sciencedirect.com/science/article/abs/pii/S0968432819303737
Papper version:info:eu-repo/semantics/acceptedVersion
licence for use:https://creativecommons.org/licenses/by/3.0/es/
Department:Història i Història de l'Art
Licence document URL:https://repositori.urv.cat/ca/proteccio-de-dades/
Thematic Areas:Zootecnia / recursos pesqueiros
Structural biology
Saúde coletiva
Química
Physics and astronomy (miscellaneous)
Physics and astronomy (all)
Odontología
Nutrição
Microscopy
Medicina veterinaria
Medicina iii
Medicina ii
Medicina i
Materials science (miscellaneous)
Materials science (all)
Materiais
Interdisciplinar
Geografía
General physics and astronomy
General medicine
General materials science
Farmacia
Ensino
Engenharias iv
Engenharias iii
Engenharias ii
Ciências biológicas iii
Ciências biológicas ii
Ciências biológicas i
Ciências ambientais
Ciências agrárias i
Cell biology
Biotecnología
Biodiversidade
Astronomia / física
Antropologia / arqueologia
Keywords:Red blood cells
Haemotaphonomy
Forensics
Ethnography
Blood smears
Archaeology
haemotaphonomy
forensics
ethnography
blood smears
archaeology
Entity:Universitat Rovira i Virgili
Record's date:2024-02-03
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