Repositori institucional URV
| URV's Author/s: | Blanco Pérez, Jordi |
| Author, as appears in the article.: | Loriot A; Van tongelen A; Blanco J; Klaessens S; Cannuyer J; van Baren N; Decottignies A; De Smet C |
| Author's mail: | jordi.blanco@urv.cat |
| Author identifier: | 0000-0001-8016-0984 |
| Journal publication year: | 2014 |
| Publication Type: | Journal Publications |
| ISSN: | 15592294 |
| APA: | Loriot A; Van tongelen A; Blanco J; Klaessens S; Cannuyer J; van Baren N; Decottignies A; De Smet C (2014). A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors. Epigenetics, 9(8), 1163-1171. DOI: 10.4161/epi.29628 |
| Papper original source: | Epigenetics. 9 (8): 1163-1171 |
| Abstract: | Genome hypomethylation is a common epigenetic alteration in human tumors, where it often leads to aberrant activation of a group of germline-specific genes, commonly referred to as cancer-germline genes. The cellular functions and tumor promoting potential of these genes remain, however, largely uncertain. Here, we report identification of a novel cancer-germline transcript (CT-GABRA3) displaying DNA hypomethylation-dependent activation in various tumors, including melanoma and lung carcinoma. Importantly, CT-GABRA3 harbors a microRNA (miR-105), which has recently been identified as a promoter of cancer metastasis by its ability to weaken vascular endothelial barriers following exosomal secretion. CT-GABRA3 also carries a microRNA (miR-767) with predicted target sites in TET1 and TET3, two members of the fen-eleven-franslocation family of tumor suppressor genes, which are involved in the conversion of 5-methylcytosines to 5-hydroxymethylcytosines (5hmC) in DNA. Decreased TET activity is a hallmark of cancer; here, we provide evidence that aberrant activation of miR-767 contributes to this phenomenon. We demonstrate that miR-767 represses TET1/3 mRNA and protein expression and regulates genomic 5hmC levels. Additionally, we show that high CT-GABRA3 transcription correlates with reduced TET1 mRNA levels in vivo in lung tumors. Together, our study identified a cancer-germline gene that produces microRNAs with oncogenic potential. Moreover, our data indicate that DNA hypomethylation in tumors can contribute to reduced 5hmC levels via activation of a TET-targeting microRNA. © 2014 Landes Bioscience. |
| Article's DOI: | 10.4161/epi.29628 |
| Link to the original source: | https://www.tandfonline.com/doi/full/10.4161/epi.29628 |
| Papper version: | info:eu-repo/semantics/publishedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Ciències Mèdiques Bàsiques |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Saúde coletiva Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina i Genetics & heredity Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cancer research Biotecnología Biochemistry & molecular biology |
| Keywords: | Tet genes Micrornas Dna methylation Cancer-germline or cancer-testis genes Cancer epigenetics tet genes dna methylation cancer-germline or cancer-testis genes cancer epigenetics |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2024-09-07 |
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