Repositori institucional URV
| URV's Author/s: | Folch Lopez, Jaume / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier |
| Author, as appears in the article.: | Patraca I; Martínez N; Busquets O; Martí A; Pedrós I; Beas-Zarate C; Marin M; Ettcheto M; Sureda F; Auladell C; Camins A; Folch J |
| Author's mail: | jaume.folch@urv.cat francesc.sureda@urv.cat |
| Author identifier: | 0000-0002-5051-8858 0000-0002-7968-3929 |
| Journal publication year: | 2017 |
| Publication Type: | Journal Publications |
| ISSN: | 17341140 |
| APA: | Patraca I; Martínez N; Busquets O; Martí A; Pedrós I; Beas-Zarate C; Marin M; Ettcheto M; Sureda F; Auladell C; Camins A; Folch J (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005 |
| Papper original source: | Pharmacological Reports. 69 (3): 409-418 |
| Abstract: | © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production. |
| Article's DOI: | 10.1016/j.pharep.2016.12.005 |
| Link to the original source: | https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub |
| Papper version: | info:eu-repo/semantics/acceptedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Pharmacology & pharmacy Pharmacology Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología |
| Keywords: | P38 mapk Leptin Inos Glial cells Cytochrome-c Caspase-3 leptin inos glial cells cytochrome-c caspase-3 |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2024-09-07 |
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