Repositori institucional URV
| URV's Author/s: | De Lamo Castellvi, Silvia |
| Author, as appears in the article.: | Hackshaw, Kevin V; Yao, Siyu; Bao, Haona; Castellvi, Silvia de Lamo; Aziz, Rija; Nuguri, Shreya Madhav; Yu, Lianbo; Osuna-Diaz, Michelle M; Brode, W Michael; Sebastian, Katherine R; Giusti, M Monica; Rodriguez-Saona, Luis |
| Author's mail: | silvia.delamo@urv.cat silvia.delamo@urv.cat |
| Author identifier: | 0000-0002-5261-6806 0000-0002-5261-6806 |
| Journal publication year: | 2023 |
| Publication Type: | Journal Publications |
| APA: | Hackshaw, Kevin V; Yao, Siyu; Bao, Haona; Castellvi, Silvia de Lamo; Aziz, Rija; Nuguri, Shreya Madhav; Yu, Lianbo; Osuna-Diaz, Michelle M; Brode, W M (2023). Metabolic Fingerprinting for the Diagnosis of Clinically Similar Long COVID and Fibromyalgia Using a Portable FT-MIR Spectroscopic Combined with Chemometrics. Biomedicines, 11(10), 2704-. DOI: 10.3390/biomedicines11102704 |
| Paper original source: | Biomedicines. 11 (10): 2704- |
| Abstract: | Post Acute Sequelae of SARS-CoV-2 infection (PASC or Long COVID) is characterized by lingering symptomatology post-initial COVID-19 illness that is often debilitating. It is seen in up to 30–40% of individuals post-infection. Patients with Long COVID (LC) suffer from dysautonomia, malaise, fatigue, and pain, amongst a multitude of other symptoms. Fibromyalgia (FM) is a chronic musculoskeletal pain disorder that often leads to functional disability and severe impairment of quality of life. LC and FM share several clinical features, including pain that often makes them indistinguishable. The aim of this study is to develop a metabolic fingerprinting approach using portable Fourier-transform mid-infrared (FT-MIR) spectroscopic techniques to diagnose clinically similar LC and FM. Blood samples were obtained from LC (n = 50) and FM (n = 50) patients and stored on conventional bloodspot protein saver cards. A semi-permeable membrane filtration approach was used to extract the blood samples, and spectral data were collected using a portable FT-MIR spectrometer. Through the deconvolution analysis of the spectral data, a distinct spectral marker at 1565 cm−1 was identified based on a statistically significant analysis, only present in FM patients. This IR band has been linked to the presence of side chains of glutamate. An OPLS-DA algorithm created using the spectral region 1500 to 1700 cm−1 enabled the classification of the spectra into their corresponding classes (Rcv > 0.96) with 100% accuracy and specificity. This high-throughput approach allows unique metabolic signatures associated with LC and FM to be identified, allowing these conditions to be distinguished and implemented for in-clinic diagnostics, which is crucial to guide future therapeutic approaches. |
| Article's DOI: | 10.3390/biomedicines11102704 |
| Link to the original source: | https://www.mdpi.com/2227-9059/11/10/2704 |
| Paper version: | info:eu-repo/semantics/publishedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Enginyeria Química |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Pharmacology & pharmacy Medicine, research & experimental Medicine (miscellaneous) General biochemistry,genetics and molecular biology Ciencias sociales Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Biochemistry & molecular biology |
| Keywords: | Post-acute sequelae of sars-cov-2 (pasc)/long covid Metabolic fingerprinting In-clinic disease diagnostics Fibromyalgia Chemometrics Blood |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2025-02-19 |
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