Repositori institucional URV
| Author: | Boutureira Martín, Omar |
| Document name: | Fluoroglycoproteins by Copper-Free Strain-Promoted Azide–Alkyne Cycloaddition |
| Book publication year: | 2020 |
| Keywords: | Clean water and sanitation |
| Abstract: | This chapter describes a reliable two-step, metal-free protocol for the preparation of well-defined fluoroglycoproteins. It starts with a first alkylation step to chemoselectively install strained alkyne handles at cysteine residues followed by a second strain-promoted azide–alkyne cycloaddition using an inverse electron-demand Diels–Alder reaction. This proof-of-principle study that uses the apoptotic protein marker Annexin V enables the efficient metal-free incorporation of 2-deoxy-2-fluoro-glycopyranosyl azides into proteins and complements previous methods using Cu(I)-mediated azide–alkyne cycloadditions and thiol chemistry. |
| ISBN: | 978-1-0716-0720-6 |
| Document URL: | https://link.springer.com/protocol/10.1007/978-1-0716-0720-6_5 |
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