### Combined effects of GBH residues and phosphate fertilizers on endophytic microbiota and plant performance of garden strawberry
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Authors: Suni Anie Mathew, Benjamin Fuchs, Riitta Nissinen, Marjo Helander, Pere Puigbò, Kari Saikkonen, Anne Muola

Contact address: Department of Biology, University of Turku, 200014, Finland
Biodiversity Unit, University of Turku, 200014, Finland

Dataset identifier: https://doi.org/10.5061/dryad.cvdncjt7k

#### Date and geographical location of data collection
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August 2019
60°26′N, 22°10′E Ruissalo Botanical Garden, University of Turku, Turku, Finland

#### Keywords
EPSPS, Fragaria X ananassa, herbicide, roundup, endophyte, microbiota, phosphate fertilizer, plant performance

#### Language 
The sample codes used in two files for strawberry is 'Ma' and control is 'K' denoting 'Mansikka' and 'Kontrolli' in Finnish.

#### Research funding
Academy of Finland (Grant No. 324523 to BF and Grant No. 311077 to MH) and the Maj and Tor Nessling Foundation (Grant No. 201800048 to AM).

#### Data and file overview
The dataset contains three files
1) Raw data on growth and runner production
The file contains data on the number of leaves, presence or absence of flowering and length of runners produced from garden strawberry plants grown in filed divided into several plots subjected to specific treatment.

plot - field was divided to 10 alterating GBH and ctrl plots; note that half of each GBH and ctrl plot was fertilized with phosphate fertilizer							
trmt -four treatments: GBH (soil sprayed with GBH), GBH+P (soil sprayed with GBH and fertilized with phosphate fertilizer), ctrl (soil sprayed with water), P (soil sprayed with water and fertilized with phosphate fertilizer)							
leaf - number	number of leaves in August 2019 (=estimate of growth)							
flowering - whether plant flowered (=1) or not (=0)							
runner length -	length of the runners produced by the plant in August 2019 (=estimate of vegetative reproduction)						
Missing values are denoted as N/A

2) Bacterial OTU
This file contains data on the reads obtained from 16S rRNA sequencing showing the OTUs pertaining to endophytic bacterial community in garden strawberry.

Sample codes - Codes for OTU table	Ma=Mansikka=Strawberry in finnish		
Treatment - four treatments: GBH (soil sprayed with GBH), GBH+P (soil sprayed with GBH and fertilized with phosphate fertilizer), ctrl (soil sprayed with water), P (soil sprayed with water and fertilized with phosphate fertilizer)			
plot no. - field was divided to 10 alterating GBH and ctrl plots; note that half of each GBH and ctrl plot was fertilized with phosphate fertilizer			
Tissue - denotes samples taken from leaf or root tissues			

3) Fungal OTU
This file contains data on the reads obtained from ITS sequencing showing the OTUs pertaining to endophytic bacterial community in garden strawberry.

Sample codes - Codes for OTU table	Ma=Mansikka=Strawberry in finnish		
Treatment - four treatments: GBH (soil sprayed with GBH), GBH+P (soil sprayed with GBH and fertilized with phosphate fertilizer), ctrl (soil sprayed with water), P (soil sprayed with water and fertilized with phosphate fertilizer)			
plot no. - field was divided to 10 alterating GBH and ctrl plots; note that half of each GBH and ctrl plot was fertilized with phosphate fertilizer			
Tissue - denotes samples taken from leaf or root tissues		

The files were created on 2021-11-09.

#### Methodological information
The experimental methods for collection of the data is explained in the article https://doi.org/10.1093/jambio/lxad006
Strawberry plantlets grown in greenhouse were planted to the experimental fields three weeks after glyphosate-based herbicide application in June 2019. The number of leaves and length of produced runners were measured in mid-August 2019. Samples for micobiome studies were collected in August 2019 from root and leaves of garden strawberry grown in glyphosate, phosphate fertilizer and glyphosate combined with phosphate fertilizer treated fields. The tissue samples were then processed for DNA extraction, PCR and high-throughput sequencing.

#### Journal article to which the dataset is linked
https://doi.org/10.1093/jambio/lxad006

End of README