Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1

Nadal Polo, Pedro

Description:

Lupin has recently been added to the list of allergens requiring mandatory advisory labelling on foodstuffs sold in the European Union, and since December 2008 all products containing even trace amounts of lupin must be labelled correctly. Lupin globulins consist of two major globulins called α-conglutin (11S and “legumin-like”) and β-conglutin (7S and “vicilin-like”), and another additional two globulins, γ-conglutin and δ-conglutin, which are present in lower amounts. β-conglutin is the only conglutin currently included in the list of the International Union of Immunological Societies (IUIS), designated as Lup an 1. The overall objective of these PhD is the selection of aptamers that can detect this allergen. Nucleic acid aptamers are synthetic ligands selected from vast combinatorial libraries through a process referred to as SELEX – Systematic Evolution of Ligand By Exponential Enrichment. Aptamers possess unique chemical and biochemical characteristics, such as: well known chemistry and remarkable stability, moreover, aptamers can be selected against virtually any target and in non-physiological conditions. In order to achieve the overall objective, a set of subobjectives will be achieved. The first of these involves the elucidation of protocols for the selective extraction of each of the lupin α, β, γ, and δ subunits, resulting in (i) protocols that can be used for selective extraction and isolation of the lupin α, β, γ, and δ proteins from food for subsequent analysis; (ii) standards that can be used in analytical assays and tools; and (iii) target that can be used for the selection of aptamers specific to the β-conglutin subunit. The core of the work is the selection of aptamers against the allergen Lup an 1 using a SELEX procedure, as well the preparation of

Type:

info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/doctoralThesis

Contributor:

Departament d'Enginyeria Química
Universitat Rovira i Virgili.

Títol:

Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1

Language:

cat

Subject:

663/664 - Aliments i nutrició. Enologia. Olis. Greixos
577 - Bioquímica. Biologia molecular. Biofísica

Format:

application/pdf
189 p.

Creator:

Nadal Polo, Pedro

Rights:

info:eu-repo/semantics/openAccess
ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al repositori institucional de la Universitat Rovira i Virgili. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a aquest repositori (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.

Date:

2012-07-12

Publisher:

Universitat Rovira i Virgili

Subject:

663/664 - Aliments i nutrició. Enologia. Olis. Greixos
577 - Bioquímica. Biologia molecular. Biofísica

Language:

cat

Publisher:

Universitat Rovira i Virgili

Source:

TDX (Tesis Doctorals en Xarxa)

Identifier:

http://hdl.handle.net/10803/84036

Format:

application/pdf
189 p.

Keywords:

Lupin
Selex
Aptamer

Date:	2012-07-12
Departament/Institute:	Departament d'Enginyeria Química Universitat Rovira i Virgili.
Director:	O'Sullivan, Ciara
Author:	Nadal Polo, Pedro
Title:	Selection, characterisation and analytical application of dna aptamer against the anaphylactic toxic allergen, b-conglutin, lup an 1

Information