Desarrollo de un método analítico por GC-FID para la determinación de escualeno en la fracción insaponificable del aceite de oliva

Oliva López, Elvira

Belongs to TFG:SerieGeneralQ collection

TITLE:

Desarrollo de un método analítico por GC-FID para la determinación de escualeno en la fracción insaponificable del aceite de oliva - TFG:129

Handle:

https://hdl.handle.net/20.500.11797/TFG129

Student:	Oliva López, Elvira
Language:	spa
Title in original language:	Desarrollo de un método analítico por GC-FID para la determinación de escualeno en la fracción insaponificable del aceite de oliva
Title in different languages:	Development of an analytical method for the squalene detemination in the olive oil insaponifiable fraction by GC-FID
Keywords:	Squalene, method, determination
Subject:	Química
Abstract:	The olive oil squalene is a compound which is getting relevance in cosmetics. It contributes interesting properties as anti-aging effect, hydration and others. It is also important in other fields, for example, in medicine, where it is used as chemopreventive agent for some type of cancers. Clariant is involved in the cosmetic field and they produce a lot of products for cosmetics. Here is where the interest in producing squalene for cosmetics applications appears. For the time being, they are studying the possibility, and one of the means needed is a method which determinates the squalene content in samples. The aim of this project is to develop a method for the squalene determination by GC-FID. During the development of optimal conditions, a permanent distortion on the squalene peak forced to change the capillary column for another wider. This distortion was due to the capillary column saturation, probably because too much sample was being injected. Dissolving the sample made the smaller peaks disappeared. Changing the column fixed the distortion problem without dissolving the sample. The identification of the sample peaks was carried out by injecting different standards. Not all peaks have been identified by lack of standards. The choice of the appropriate solvent and internal standard was done by repeatability studies. Two solvents were tested, among which ethyl acetate gave better repeatability results, so it was chosen. Two different internal standards were tested, too. Taking into account the premises that an internal standard must accomplish, 1-octadecene proved to be the adequate for squalene determination. Once the accuracy and the linearity of the chromatograph were checked, the method accuracy and linearity were studied. Both gave optimal results, making this method useful for squalene determination. However, not all the validation parameters have been studied due to the limited time, so it would be interesting to continue optimizing the method.
Project director:	Carrasco Campos, Ariadna
Work's codirector:	Matheu Malpartida, Maribel
Department:	Química Analítica i Química Orgànica
Education area(s):	Química
Entity:	Universitat Rovira i Virgili (URV)
TFG credits:	12
Creation date in repository:	2015-02-18
Work's public defense date:	2014-06-12
Academic year:	2013-2014
Confidenciality:	No
Subject areas:	Chemistry
Access rights:	info:eu-repo/semantics/openAccess

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Type:	info:eu-repo/semantics/bachelorThesis
Títol:	Desarrollo de un método analítico por GC-FID para la determinación de escualeno en la fracción insaponificable del aceite de oliva
Contributor:	Matheu Malpartida, Maribel Carrasco Campos, Ariadna
Subject:	Química Chemistry Química Química
Date:	2014-06-12
Language:	spa
Creator:	Oliva López, Elvira
Rights:	info:eu-repo/semantics/openAccess

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