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TITLE:

Construction and characterization of Komagataella phaffii strains that produce CRL1 lipase under the control of TSA1 promoter - TFG:7943
Handle:

https://hdl.handle.net/20.500.11797/TFG7943

Student:Garcia Palomares, Lucia
Language:en
Title in original language:Construction and characterization of Komagataella phaffii strains that produce CRL1 lipase under the control of TSA1 promoter
Title in different languages:Construction and characterization of Komagataella phaffii strains that produce CRL1 lipase under the control of TSA1 promoter
Keywords:Pichia pastoris, lipase, TSA1
Subject:Bioquímica i biotecnologia
Abstract:This work explored the production of Candida rugosa lipase 1 (CRL1) in Komagataella phaffii using the Thioredoxin peroxidase promoter (TSA1), which has been reported to be inducible by alkaline pH, as an alternative to the traditional aldehyde oxidase (pAOX1) methanol-inducible system. Although higher lipase production was expected at pH 8.0, the results showed greater activity at pH 5.5. This suggests that the insertion of the genetic cassette may have altered the promoter's response. Despite not achieving induction with alkaline pH, remarkably high constitutive lipase production was obtained at pH 5.5, surpassing even the pAOX1 system in terms of efficiency and safety.
Project director:Calvo Manso, Enrique
Department:Bioquímica i Biotecnologia
Education area(s):Bioquímica i Biologia Molecular
Entity:Universitat Rovira i Virgili (URV)
Creation date in repository:2025-01-22
Work's public defense date:2024-09-13
Academic year:2023-2024
Confidenciality:Si
Subject areas:Biochemistry and biotechnology
Access rights:info:eu-repo/semantics/openAccess
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