Repositori institucional URV
| URV's Author/s: | Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña |
| Author, as appears in the article.: | Mas-Capdevila, Anna; Iglesias-Carres, Lisard; Arola-Arnal, Anna; Aragones, Gerard; Aleixandre, Amaya; Bravo, Francisca I; Muguerza, Begona |
| Author's mail: | franciscaisabel.bravo@urv.cat anna.arola@urv.cat gerard.aragones@urv.cat begona.muguerza@urv.cat |
| Author identifier: | 0000-0002-6468-3088 0000-0001-6529-1345 0000-0001-8657-5726 0000-0001-7384-8588 |
| Journal publication year: | 2019 |
| Publication Type: | Journal Publications |
| e-ISSN: | 2072-6643 |
| APA: | Mas-Capdevila, Anna; Iglesias-Carres, Lisard; Arola-Arnal, Anna; Aragones, Gerard; Aleixandre, Amaya; Bravo, Francisca I; Muguerza, Begona (2019). Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats.. Nutrients, 11(2), e225-. DOI: 10.3390/nu11020225 |
| Paper original source: | Nutrients. 11 (2): e225- |
| Abstract: | AVFQHNCQE is an antihypertensive nonapeptide obtained from a chicken foot protein hydrolysate. The present study aims to investigate the mechanisms involved in its blood pressure (BP)-lowering effect. Male (17?20 weeks old) spontaneously hypertensive rats (SHR) were used in this study. Rats were divided into two groups and orally administered water or 10 mg/kg body weight (bw) AVFQHNCQE. One hour post-administration, animals of both groups were intra-peritoneally treated with 1 mL of saline or with 1 mL of saline containing 30 mg/kg bw N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, or with 1 mL of saline containing 5 mg/kg bw indomethacin, which is an inhibitor of prostacyclin synthesis (n = 6 per group). Systolic BP was recorded before oral administration and six hours after oral administration. In an additional experiment, SHR were administered water or 10 mg/kg bw AVFQHNCQE (n = 6 per group) and sacrificed six hours post-administration to study the mechanisms underlying the peptide anti-hypertensive effect. Moreover, the relaxation caused by AVFQHNCQE in isolated aortic rings from Sprague-Dawley rats was evaluated. The BP-lowering effect of the peptide was not changed after indomethacin administration but was completely abolished by L-NAME, which demonstrates that its anti-hypertensive effect is mediated by changes in endothelium-derived NO availability. In addition, AVFQHNCQE administration downregulated aortic gene expression of the vasoconstrictor factor endothelin-1 and the endothelial major free radical producer NADPH. Moreover, while no changes in plasma ACE activity were observed after its administration, liver GSH levels were higher in the peptide-treated group than in the water group, which demonstrates that AVFQHNCQE presents antioxidant properties. |
| Article's DOI: | 10.3390/nu11020225 |
| Link to the original source: | https://www.mdpi.com/2072-6643/11/2/225 |
| Paper version: | info:eu-repo/semantics/publishedVersion |
| licence for use: | https://creativecommons.org/licenses/by/3.0/es/ |
| Department: | Bioquímica i Biotecnologia |
| Licence document URL: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Thematic Areas: | Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología |
| Keywords: | Spontaneously hypertensive rats Rna Rats, sprague-dawley Rats, inbred shr Rats Proteins Pro Peptides Oxidative stress Nitric oxide Mechanisms Malondialdehyde Male Gene expression regulation Endothelium, vascular Endothelial dysfunction Egg-white hydrolysate Cardiovascular-diseases Blood pressure Bioactivity Bioactive peptide Aorta Antihypertensive peptides Antihypertensive agents Animals Amino acid sequence Alpha-lactorphin Ace-inhibitory peptides nitric oxide endothelial dysfunction blood pressure bioactivity bioactive peptide |
| Entity: | Universitat Rovira i Virgili |
| Record's date: | 2025-02-08 |
| Journal volume: | 11 |
| Search your record at: |   |
| File | Description | Format | |
|---|---|---|---|
| DocumentPrincipal | DocumentPrincipal | application/pdf |
© 2011 Universitat Rovira i Virgili