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Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition - imarina:6389047

Autor/es de la URV:Folch Lopez, Jaume / Martí Zaragoza, Àlex / Martínez Micaelo, Nieves Beatriz / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier
Autor según el artículo:Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc; Auladell, Carme; Camins, Antoni; Folch, Jaume
Direcció de correo del autor:neus.martinez@urv.cat
alex.marti@estudiants.urv.cat
jaume.folch@urv.cat
francesc.sureda@urv.cat
Identificador del autor:0000-0002-5051-8858
0000-0002-7968-3929
Año de publicación de la revista:2017
Tipo de publicación:Journal Publications
ISSN:17341140
Referencia de l'ítem segons les normes APA:Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005
Referencia al articulo segun fuente origial:Pharmacological Reports. 69 (3): 409-418
Resumen:© 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
DOI del artículo:10.1016/j.pharep.2016.12.005
Enlace a la fuente original:https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub
Versión del articulo depositado:info:eu-repo/semantics/acceptedVersion
Acceso a la licencia de uso:https://creativecommons.org/licenses/by/3.0/es/
Departamento:Ciències Mèdiques Bàsiques
Bioquímica i Biotecnologia
URL Documento de licencia:https://repositori.urv.cat/ca/proteccio-de-dades/
Áreas temáticas:Zootecnia / recursos pesqueiros
Saúde coletiva
Química
Psicología
Pharmacology & pharmacy
Pharmacology
Odontología
Nutrição
Medicine (miscellaneous)
Medicina veterinaria
Medicina iii
Medicina ii
Medicina i
Interdisciplinar
Farmacia
Educação física
Ciências biológicas iii
Ciências biológicas ii
Ciências biológicas i
Ciências ambientais
Biotecnología
Palabras clave:Tumor necrosis factor-alpha
P38 mitogen-activated protein kinases
P38 mapk
Nitric oxide
Neuroglia
Mice, inbred c57bl
Mice
Map kinase signaling system
Leptin
Interleukin-1beta
Interferon-gamma
Inos
Inflammation
Glial cells
Disease models, animal
Cytokines
Cytochrome-c
Cells, cultured
Cell survival
Caspase-3
Apoptosis
Anti-inflammatory agents
Animals
leptin
inos
glial cells
cytochrome-c
caspase-3
Entidad:Universitat Rovira i Virgili
Fecha de alta del registro:2024-10-19
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