Repositori institucional URV
| Autor/s de la URV: | Fernandez Veledo, Sonia / Maymo Masip, Elsa / Megía Colet, Ana / MILLAN SCHEIDING, MONICA / Serena Perelló, Carolina / Vendrell Ortega, Juan José |
| Autor segons l'article: | Madeira, Ana; Serena, Carolina; Ejarque, Miriam; Maymo-Masip, Elsa; Millan, Monica; Navarro-Ruiz, M. Carmen; Guzman-Ruiz, Rocio; Malagon, Maria M.; Espin, Eloy; Marti, Marc; Menacho, Margarita; Megia, Ana; Vendrell, Joan; Fernandez-Veledo, Sonia; |
| Adreça de correu electrònic de l'autor: | sonia.fernandez@urv.cat carolina.serena@urv.cat elsa.maymo@urv.cat ana.megia@urv.cat juanjose.vendrell@urv.cat |
| Identificador de l'autor: | 0000-0003-2906-3788 0000-0002-9133-3120 0000-0002-5101-9452 0000-0002-6994-6115 |
| Any de publicació de la revista: | 2021 |
| Tipus de publicació: | Journal Publications |
| Referència de l'ítem segons les normes APA: | Madeira, Ana; Serena, Carolina; Ejarque, Miriam; Maymo-Masip, Elsa; Millan, Monica; Navarro-Ruiz, M. Carmen; Guzman-Ruiz, Rocio; Malagon, Maria M.; Es (2021). Crohn's Disease Increases the Mesothelial Properties of Adipocyte Progenitors in the Creeping Fat. International Journal Of Molecular Sciences, 22(8), -. DOI: 10.3390/ijms22084292 |
| Referència a l'article segons font original: | International Journal Of Molecular Sciences. 22 (8): |
| Resum: | Our understanding of the interplay between human adipose tissue and the immune system is limited. The mesothelium, an immunologically active structure, emerged as a source of visceral adipose tissue. After investigating the mesothelial properties of human visceral and subcutaneous adipose tissue and their progenitors, we explored whether the dysfunctional obese and Crohn's disease environments influence the mesothelial/mesenchymal properties of their adipocyte precursors, as well as their ability to mount an immune response. Using a tandem transcriptomic/proteomic approach, we evaluated the mesothelial and mesenchymal expression profiles in adipose tissue, both in subjects covering a wide range of body-mass indexes and in Crohn's disease patients. We also isolated adipose tissue precursors (adipose-derived stem cells, ASCs) to assess their mesothelial/mesenchymal properties, as well as their antigen-presenting features. Human visceral tissue presented a mesothelial phenotype not detected in the subcutaneous fat. Only ASCs from mesenteric adipose tissue, named creeping fat, had a significantly higher expression of the hallmark mesothelial genes mesothelin (MSLN) and Wilms' tumor suppressor gene 1 (WT1), supporting a mesothelial nature of these cells. Both lean and Crohn's disease visceral ASCs expressed equivalent surface percentages of the antigen-presenting molecules human leucocyte antigen-DR isotype (HLA-DR) and CD86. However, lean-derived ASCs were predominantly HLA-DR (dim), whereas in Crohn's disease, the HLA-DR (bright) subpopulation was increased 3.2-fold. Importantly, the mesothelial-enriched Crohn's disease precursors activated CD4(+) T-lymphocytes. Our study evidences a mesothelial signature in the creeping fat of Crohn's disease patients and its progenitor cells, the latter being able to present antigens and orchestrate an immune response. |
| DOI de l'article: | 10.3390/ijms22084292 |
| Enllaç font original: | https://www.mdpi.com/1422-0067/22/8/4292 |
| Versió de l'article dipositat: | info:eu-repo/semantics/publishedVersion |
| Accès a la llicència d'ús: | https://creativecommons.org/licenses/by/3.0/es/ |
| Departament: | Medicina i Cirurgia |
| URL Document de llicència: | https://repositori.urv.cat/ca/proteccio-de-dades/ |
| Àrees temàtiques: | Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física |
| Paraules clau: | Wt1 protein Tumor suppressor gene Transcriptomics Transcriptome Subcutaneous fat Stem cells Stem cell S disease Proteomics Protein expression Procedures Phenotype Pathology Obesity Middle aged Metabolism Mesothelium Mesothelin Mesenteric fat Mesenchyme cell Male Major clinical study Intra-abdominal fat Immunophenotyping Immunology Immune response Humans Human cell Human Hla dr antigen Gene expression profiling Gene expression Female Crohn’ Crohn’s disease Crohn disease Creeping fat Controlled study Computational biology Cohort analysis Cell subpopulation Cd86 antigen Cd4+ t lymphocyte Body mass Biomarkers Biology Biological marker Article Antigen-presenting cells Antigen presenting cell Antigen presentation Adult Adolescent Adipose-derived stem cells Adipose tissue Adipose derived stem cell Adipocyte |
| Entitat: | Universitat Rovira i Virgili |
| Data d'alta del registre: | 2024-07-27 |
| Volum de revista: | 22 |
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| DocumentPrincipal | DocumentPrincipal | application/pdf |
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