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Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp. - imarina:9280246

Autor/s de la URV:Fernández Bravo, Ana / Figueras Salvat, María Josefa
Autor segons l'article:Fernández-Bravo A; Figueras MJ
Adreça de correu electrònic de l'autor:ana.fernandez@urv.cat
mariajose.figueras@urv.cat
Identificador de l'autor:0000-0001-7637-5958
0000-0002-2268-8980
Any de publicació de la revista:2022
Tipus de publicació:Journal Publications
Referència de l'ítem segons les normes APA:Fernández-Bravo A; Figueras MJ (2022). Immune Response of the Monocytic Cell Line THP-1 Against Six Aeromonas spp.. Frontiers In Immunology, 13(), -. DOI: 10.3389/fimmu.2022.875689
Referència a l'article segons font original:Frontiers In Immunology. 13
Resum:Aeromonas are autochthonous bacteria of aquatic environments that are considered to be emerging pathogens to humans, producing diarrhea, bacteremia, and wound infections. Genetic identification shows that 95.4% of the strains associated with clinical cases correspond to the species Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). However, few studies have investigated the human immune response against some Aeromonas spp. such as A. hydrophila, Aeromonas salmonicida, and A. veronii. The present study aimed to increase the knowledge about the innate human immune response against six Aeromonas species, using, for the first time, an in vitro infection model with the monocytic human cell line THP-1, and to evaluate the intracellular survival, the cell damage, and the expression of 11 immune-related genes (TLR4, TNF-α, CCL2, CCL20, JUN, RELA, BAX, TP53, CASP3, NLRP3, and IL-1β). Transcriptional analysis showed an upregulated expression of a variety of the monocytic immune-related genes, with a variable response depending upon the Aeromonas species. The species that produced the highest cell damage, independently of the strain origin, coincidentally induced a higher expression of immune-related genes and corresponded to the more prevalent clinical species A. dhakensis, A. veronii, and A. caviae. Additionally, monocytic cells showed an overexpression of the apoptotic and pyroptotic genes involved in cell death after A. dhakensis, A. caviae, and Aeromonas media infection. However, the apoptosis route seemed to be the only way of producing cell damage and death in the case of the species Aeromonas piscicola and Aeromonas jandaei, while A. veronii apparently only used the pyroptosis route.
DOI de l'article:10.3389/fimmu.2022.875689
Enllaç font original:https://www.frontiersin.org/articles/10.3389/fimmu.2022.875689/full
Versió de l'article dipositat:info:eu-repo/semantics/publishedVersion
Accès a la llicència d'ús:https://creativecommons.org/licenses/by/3.0/es/
Departament:Ciències Mèdiques Bàsiques
URL Document de llicència:https://repositori.urv.cat/ca/proteccio-de-dades/
Àrees temàtiques:Zootecnia / recursos pesqueiros
Saúde coletiva
Química
Odontología
Nutrição
Medicina veterinaria
Medicina iii
Medicina ii
Medicina i
Interdisciplinar
Immunology and allergy
Immunology
Farmacia
Engenharias iii
Engenharias ii
Ciências biológicas iii
Ciências biológicas ii
Ciências biológicas i
Ciência de alimentos
Biotecnología
Biodiversidade
Paraules clau:Monocytic cells
Intracellular survival
Immune-related genes
Cell damage
Aeromonasspp
Entitat:Universitat Rovira i Virgili
Data d'alta del registre:2024-09-07
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