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Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas - imarina:9295157

Autor/s de la URV:Figueras Salvat, María Josefa
Autor segons l'article:Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte;
Adreça de correu electrònic de l'autor:mariajose.figueras@urv.cat
Identificador de l'autor:0000-0002-2268-8980
Any de publicació de la revista:2023
Tipus de publicació:Journal Publications
Referència de l'ítem segons les normes APA:Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte; (2023). Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas. Pathogens, 12(3), -. DOI: 10.3390/pathogens12030405
Referència a l'article segons font original:Pathogens. 12 (3):
Resum:Background: Polymicrobial infections are complex infections associated with worse outcomes compared to monomicrobial infections. We need simple, fast, and cost-effective animal models to assess their still poorly known pathogenesis. Methods: We developed a Drosophila melanogaster polymicrobial infection model for opportunistic pathogens and assessed its capacity to discriminate the effects of bacterial mixtures taken from cases of human polymicrobial infections by Aeromonas strains. A systemic infection was obtained by needle pricking the dorsal thorax of the flies, and the fly survival was monitored over time. Different lineages of the flies were infected by a single strain or paired strains (strain ratio 1:1). Results: Individual strains killed more than 80% of the flies in 20 h. The course of infection could be altered with a microbial mix. The model could distinguish between the diverse effects (synergistic, antagonistic, and no difference) that resulted in a milder, more severe, or similar infection, depending on the paired strain considered. We then investigated the determinants of the effects. The effects were maintained in deficient fly lineages for the main signaling pathways (Toll deficient and IMD deficient), which suggests an active microbe/microbe/host interaction. Conclusion: These results indicate that the D. melanogaster systemic infection model is consistent with the study of polymicrobial infection.
DOI de l'article:10.3390/pathogens12030405
Enllaç font original:https://www.mdpi.com/2076-0817/12/3/405
Versió de l'article dipositat:info:eu-repo/semantics/publishedVersion
Accès a la llicència d'ús:https://creativecommons.org/licenses/by/3.0/es/
Departament:Ciències Mèdiques Bàsiques
URL Document de llicència:https://repositori.urv.cat/ca/proteccio-de-dades/
Àrees temàtiques:Molecular biology
Microbiology (medical)
Microbiology
Infectious diseases
Immunology and microbiology (miscellaneous)
Immunology and microbiology (all)
Immunology and allergy
General immunology and microbiology
Farmacia
Paraules clau:Pseudomonas-aeruginosa
Polymicrobial infection
Opportunistic pathogen
Microbial interference
Lessons
Infection model
Host
Drosophila melanogaster
Disease
Bacteria
Aeromonas
Entitat:Universitat Rovira i Virgili
Data d'alta del registre:2024-08-03
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