Articles producció científica> Enginyeria Química

The bioeffects resulting from prokaryotic cells and yeast being exposed to an 18 GHz electromagnetic field

  • Dades identificatives

    Identificador: PC:1820
    Autors:
    Vladimir BaulinThe Hong Phong NguyenVy T. H. PhamSong Ha NguyenRodney J. CroftBrian PhillipsRussell J. CrawfordElena P. Ivanova
    Resum:
    The mechanisms by which various biological effects are triggered by exposure to an electromagnetic field are not fully understood and have been the subject of debate. Here, the effects of exposing typical representatives of the major microbial taxa to an 18 GHz microwave electromagnetic field (EMF)were studied. It appeared that the EMF exposure induced cell permeabilisation in all of the bacteria and yeast studied, while the cells remained viable (94% throughout the exposure), independent of the differences in cell membrane fatty acid and phospholipid composition. The resulting cell permeabilisation was confirmed by detection of the uptake of propidium iodine and 23 nm fluorescent silica nanospheres using transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). Upon EMF exposure, the bacterial cell membranes are believed to become permeable through quasi-endocytosis processes. The dosimetry analysis revealed that the EMF threshold level required to induce the uptake of the large (46 nm) nanopsheres was between three and six EMF doses, with a specific absorption rate (SAR) of 3 kW/kg and 5 kW/kg per exposure, respectively, depending on the bacterial taxa being studied. It is suggested that the taxonomic affiliation and lipid composition (e.g. the presence of phosphatidyl-glycerol and/or pentadecanoic fatty acid) may affect the extent of uptake of the large nanospheres (46 nm). Multiple 18 GHz EMF exposures over a one-hour period induced periodic anomalous increases in the cell growth behavior of two Staphylococcus aureus strains, namely ATCC 25923 and CIP 65.8T.
  • Altres:

    Autor segons l'article: Vladimir Baulin; The Hong Phong Nguyen; Vy T. H. Pham; Song Ha Nguyen; Rodney J. Croft; Brian Phillips; Russell J. Crawford; Elena P. Ivanova
    Departament: Enginyeria Química
    Autor/s de la URV: BAULIN ., VLADIMIR; The Hong Phong Nguyen; Vy T. H. Pham; Song Ha Nguyen; Rodney J. Croft; Brian Phillips; Russell J. Crawford; Elena P. Ivanova
    Resum: The mechanisms by which various biological effects are triggered by exposure to an electromagnetic field are not fully understood and have been the subject of debate. Here, the effects of exposing typical representatives of the major microbial taxa to an 18 GHz microwave electromagnetic field (EMF)were studied. It appeared that the EMF exposure induced cell permeabilisation in all of the bacteria and yeast studied, while the cells remained viable (94% throughout the exposure), independent of the differences in cell membrane fatty acid and phospholipid composition. The resulting cell permeabilisation was confirmed by detection of the uptake of propidium iodine and 23 nm fluorescent silica nanospheres using transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). Upon EMF exposure, the bacterial cell membranes are believed to become permeable through quasi-endocytosis processes. The dosimetry analysis revealed that the EMF threshold level required to induce the uptake of the large (46 nm) nanopsheres was between three and six EMF doses, with a specific absorption rate (SAR) of 3 kW/kg and 5 kW/kg per exposure, respectively, depending on the bacterial taxa being studied. It is suggested that the taxonomic affiliation and lipid composition (e.g. the presence of phosphatidyl-glycerol and/or pentadecanoic fatty acid) may affect the extent of uptake of the large nanospheres (46 nm). Multiple 18 GHz EMF exposures over a one-hour period induced periodic anomalous increases in the cell growth behavior of two Staphylococcus aureus strains, namely ATCC 25923 and CIP 65.8T.
    Grup de recerca: Molecular simulation I: Complex Systems
    Àrees temàtiques: Chemical engineering Ingeniería química Enginyeria química
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1932-6203
    Identificador de l'autor: 0000-0003-2086-4271; n/a; n/a; n/a; n/a; n/a; n/a; n/a
    Data d'alta del registre: 2016-09-13
    Volum de revista: 11
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0158135
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1371/journal.pone.0158135
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Pàgina inicial: Art.num. e158135
    Tipus de publicació: Article Artículo Article
  • Paraules clau:

    Electromagnetic field
    Prokaryotic cell
    Procariotes
    Camps electromagnètics
    Llevats
    Chemical engineering
    Ingeniería química
    Enginyeria química
    1932-6203
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