Autor segons l'article: Rius, F. Xavier; Khan, M. Azizur R. ; Aires Cardoso, Ana Rita; Sales, M. Goreti F.; Merino, Susana; Tomás, Juan M. ; Riu, J
Departament: Química Analítica i Química Orgànica
Autor/s de la URV: RIUS FERRÚS, FRANCISCO JAVIER; Khan, M. Azizur R. ; Aires Cardoso, Ana Rita; Sales, M. Goreti F.; Merino, Susana; Tomás, Juan M. ; RIU RUSELL, JORDI
Resum: In this paper for the first time we successfully detect bacterial flagellar filaments from Proteus mirabilis using molecularly imprinted artificial receptors. These receptors acted as a sensing layer of the biosensors, assembled by imprinting flagellar proteins onto a polymeric backbone of electropolymerized phenol. In short, flagellar filaments were absorbed onto a carbon support, phenol was electropolymerized around it through the carbon conductive matrix to create the protein molecular molds, and finally the flagellar proteins were removed by enzymatic and electrochemical action. Each removed flagellar protein gave rise to an imprinted site with eventual rebinding ability. Electrical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed to measure the interaction of flagellar filaments with the sensing layer assembled on commercial screen-printed electrodes, providing low detection limits, high precision and selectivity toward the targeted protein. The detection limit was 0.7 ng/mL by EIS and 0.9 ng/mL by SWV. The artificial receptors were further assembled on home-made paper-printed electrodes, with the three-electrode system printed on a paper substrate, offering the possibility of detecting flagellar filaments at as low as 0.6 ng/mL with a disposable and cost-effective portable device. To the best of our knowledge this is the first sensing device where molecularly imprinted artificial receptors are tailored on home-made electrode based on paper substrates with three electrodes assembled together, which is a suitable approach for the fabrication of easy and cost-effective tailored electrodes.
Grup de recerca: Grup de Quimiometria, Qualimetria i Nanosensors
Àrees temàtiques: Química Química Chemistry
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0925-4005
Identificador de l'autor: 0000-0003-4830-1366; ; ; ; ; ; 0000-0001-5823-9223
Data d'alta del registre: 2018-04-26
Pàgina final: 741
Volum de revista: 244
Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0925400517300187?via%3Dihub
Programa de finançament: plan; CTQ2010-18717 altres; PhD Fellowship; Gencat FI; 2013FI_B00842
DOI de l'article: 10.1016/j.snb.2017.01.018
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Pàgina inicial: 732
Tipus de publicació: Article Artículo Article
Repositori URV