Articles producció científica> Química Física i Inorgànica

Microfluidic device with dual-channel fluorescence acquisition for quantfication/identification of cancer cells

  • Dades identificatives

    Identificador: PC:3254
    Autors:
    Aguiló, M.Pedrol, E.Martínez, J.García-Algar, M.Nazarenus, M.Guerrini, L.Garcia-Rico, E.Alvarez-Puebla, R.Díaz, F.Massons, J.
    Resum:
    An optofluidic device for cell discrimination with two independent interrogation regions is presented. Pumping light coupling to the device and cells’ fluorescence extraction from the two interrogation zones is accomplished employing optical fibers embedded in said optofluidic chip. To test the reliability of this device AU-565 cells -expressing EpCAM and HER2 receptors- and RAMOS cells were mixed in a controlled manner, confined inside an hydrodynamic focused flow in the microfluidic chip and detected individually to be later discriminated as positive (signal re- ception from fluorescently labeled antibodies from the AU-565 cells) or negative events (RAMOS cells). A correlation analysis is performed over the two intensity versus time signals obtained at each of the two interrogation zones. A post-processing analysis permits a peak-to-peak pairing between the two output signals, being the detected peaks at each channel events related to the passing of a single cell through an interrogation region. Said method reduces the influence of noise on the overall data and allows to better discern actual cells’ characteristic intensity bursts from noise without the need of digitally aided signal processing.
  • Altres:

    Autor segons l'article: Aguiló, M. ; Pedrol, E. ; Martínez, J. ; García-Algar, M. ; Nazarenus, M. ; Guerrini, L. ; Garcia-Rico, E. ; Alvarez-Puebla, R. ; Díaz, F. ; Massons, J.
    Departament: Química Física i Inorgànica
    Autor/s de la URV: AGUILÓ DÍAZ, MAGDALENA; Pedrol, E. ; Martínez, J. ; García-Algar, M. ; Nazarenus, M. ; GUERRINI , LUCA; Garcia-Rico, E. ; ALVAREZ PUEBLA, RAMON ANGEL; DÍAZ GONZÁLEZ, FRANCISCO MANUEL; MASONS BOSCH, JAIME
    Paraules clau: cancer cells dual-channel fluorescence acquisition Microfluidic devices
    Resum: An optofluidic device for cell discrimination with two independent interrogation regions is presented. Pumping light coupling to the device and cells’ fluorescence extraction from the two interrogation zones is accomplished employing optical fibers embedded in said optofluidic chip. To test the reliability of this device AU-565 cells -expressing EpCAM and HER2 receptors- and RAMOS cells were mixed in a controlled manner, confined inside an hydrodynamic focused flow in the microfluidic chip and detected individually to be later discriminated as positive (signal re- ception from fluorescently labeled antibodies from the AU-565 cells) or negative events (RAMOS cells). A correlation analysis is performed over the two intensity versus time signals obtained at each of the two interrogation zones. A post-processing analysis permits a peak-to-peak pairing between the two output signals, being the detected peaks at each channel events related to the passing of a single cell through an interrogation region. Said method reduces the influence of noise on the overall data and allows to better discern actual cells’ characteristic intensity bursts from noise without the need of digitally aided signal processing.
    Grup de recerca: Física i Cristal·lografia de Nanomaterials Grupo de Plasmonica y Ultradetección Física i Cristal.lografia de Materials
    Àrees temàtiques: Physics Física Física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1613-4982
    Identificador de l'autor: 0000-0001-6130-9579; ; ; ; ; 0000-0002-2925-1562; ; 0000-0003-4770-5756; 0000-0003-4581-4967; 0000-0003-4325-6084
    Data d'alta del registre: 2018-06-04
    Volum de revista: 21
    Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
    Enllaç font original: https://link.springer.com/article/10.1007/s10404-017-2015-3
    Programa de finançament: altres; Grupos consolidados; 2014SGR480 altres; Grupos consolidados; 2014SGR1358 plan; Retos; CTQ2014-59808R plan; Retos; TEC2014-55948-R plan; Retos; MAT2013-47395- C4-4-R plan; Retos; MAT2016- 75716-C2-1-R (AEI/FEDER, UE)
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI de l'article: 10.1007/s10404-017-2015-3
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2017
    Pàgina inicial: 187
    Tipus de publicació: Article Artículo Article
  • Paraules clau:

    Cristal·lografia
    Física
    Materials nanoestructurats
    cancer cells
    dual-channel fluorescence acquisition
    Microfluidic devices
    Physics
    Física
    Física
    1613-4982
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