Autor segons l'article: Ferna?dez-Silva F; Lackner M; Capilla J; Mayayo E; Sutton D; Castanheira M; Fothergill A; Lass-Flörl C; Guarro J
Departament: Ciències Mèdiques Bàsiques
Autor/s de la URV: Capilla Luque, Javier / FERNÁNDEZ SILVA, FABIOLA VANESSA / Guarro Artigas, Josep / Mayayo Artal, Emilio Manuel
Paraules clau: Therapy Surveillance Resistance Micafungin Management Liposomal amphotericin-b Identification Epidemiology Echinocandin Candida glabrata Anidulafungin
Resum: It has been argued that the in vitro activity of caspofungin (CSP) is not a good predictor of the outcome of echinocandin treatment in vivo. We evaluated the in vitro activity of CSP and the presence of FKS mutations in the hot spot 1 (HS1) region of the FKS1 and FKS2 genes in 17 Candida glabrata strains with a wide range of MICs. The efficacy of CSP against systemic infections from each of the 17 strains was evaluated in a murine model. No HS1 mutations were found in the eight strains showing MICs for CSP of <= 0.5 mu g/ml, but they were present in eight of the nine strains with MICs of >= 1 mu g/ml, i.e., three in the FKS1 gene and five in the FKS2 gene. CSP was effective for treating mice infected with strains with MICs of <= 0.5 mu g/ml, showed variable efficacy in animals challenged with strains with MICs of 1 mu g/ml, and did not work in those with strains with MICs of 1 mu g/ml. In addition, mutations, including one reported for the first time, were found outside the HS1 region in the FKS2 gene of six strains with different MICs, but their presence did not influence drug efficacy. The in vitro activity of CSP was compared with that of another echinocandin, anidulafungin, suggesting that the MICs of both drugs, as well as mutations in the HS1 regions of the FKS1 and FKS2 genes, are predictive of outcome.
Àrees temàtiques: Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Infectious diseases Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: josep.guarro@urv.cat javier.capilla@urv.cat
Identificador de l'autor: 0000-0002-7839-7568 0000-0002-0765-6403
Data d'alta del registre: 2023-12-16
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://journals.asm.org/doi/10.1128/aac.02666-13
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Antimicrobial Agents And Chemotherapy. 58 (7): 3646-3649
Referència de l'ítem segons les normes APA: Ferna?dez-Silva F; Lackner M; Capilla J; Mayayo E; Sutton D; Castanheira M; Fothergill A; Lass-Flörl C; Guarro J (2014). In vitro antifungal susceptibility of Candida glabrata to caspofungin and the presence of FKS mutations correlate with treatment response in an immunocompromised murine model of invasive infection.. Antimicrobial Agents And Chemotherapy, 58(7), 3646-3649. DOI: 10.1128/AAC.02666-13
DOI de l'article: 10.1128/AAC.02666-13
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2014
Tipus de publicació: Journal Publications
Repositori URV