Autor segons l'article: García-Fuentes E; Santiago-Fernández C; Gutiérrez-Repiso C; Mayas M; Oliva-Olivera W; Coín-Aragüez L; Alcaide J; Ocaña-Wilhelmi L; Vendrell J; Tinahones F; Garrido-Sánchez L
Departament: Medicina i Cirurgia
Autor/s de la URV: Vendrell Ortega, Juan José
Paraules clau: Suppression Obesity Morbid obesity Mechanisms Insulin-resistance Insulin resistance Hypoxia Hif-1aα Hif-1a? Hif-1 alpha Dysregulation Disease Blood-flow Binding Atp citrate-lyase Adipose tissue Adipocytes
Resum: Background: A key role for HIF-1 alpha in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue.
Methods: We studied HIF-1 alpha mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis.
Results: HIF-1 alpha was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1 alpha mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1 alpha mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1 alpha mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1 alpha mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-gamma. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-gamma, SREBP-1c, ACLY, ACC1 and FASN mRNA expression.
Conclusions: Morbidly obese subjects have a higher level of VAT HIF-1 alpha. Postprandial status is associated with an increase in HIF-1 alpha mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT.
Àrees temàtiques: Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar General medicine General biochemistry,genetics and molecular biology Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: juanjose.vendrell@urv.cat
Identificador de l'autor: 0000-0002-6994-6115
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-015-0732-5
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Journal Of Translational Medicine. 13 (1):
Referència de l'ítem segons les normes APA: García-Fuentes E; Santiago-Fernández C; Gutiérrez-Repiso C; Mayas M; Oliva-Olivera W; Coín-Aragüez L; Alcaide J; Ocaña-Wilhelmi L; Vendrell J; Tinahon (2015). Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue. Journal Of Translational Medicine, 13(1), -. DOI: 10.1186/s12967-015-0732-5
DOI de l'article: 10.1186/s12967-015-0732-5
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2015
Tipus de publicació: Journal Publications