Autor segons l'article: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M
Departament: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/s de la URV: Yanes Torrado, Óscar
Resum: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffinembedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where metabolites were extracted in a manner that preserves tissue architecture. Despite the fact that selected metabolites were not retained after processing, global metabolic profiles obtained from FFPE can be used to predict biologic states and study biologic pathways. These results pave the way for metabolomics-based biomarker discovery/validation utilizing retrospective and clinically annotated FFPE collections.
Àrees temàtiques: Oncology Odontología Molecular biology Medicine (all) Medicina iii Medicina ii Medicina i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Cancer research Biotecnología
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: oscar.yanes@urv.cat
Identificador de l'autor: 0000-0003-3695-7157
Data d'alta del registre: 2023-07-31
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://mcr.aacrjournals.org/content/15/4/439
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Molecular Cancer Research. 15 (4): 439-447
Referència de l'ítem segons les normes APA: Cacciatore S; Zadra G; Bango C; Penney K; Tyekucheva S; Yanes O; Loda M (2017). Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues. Molecular Cancer Research, 15(4), 439-447. DOI: 10.1158/1541-7786.MCR-16-0262
DOI de l'article: 10.1158/1541-7786.MCR-16-0262
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Tipus de publicació: Journal Publications
Repositori URV