Autor segons l'article: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.
Departament: Química Analítica i Química Orgànica
Autor/s de la URV: Serra Albet, Maria Angels
Paraules clau: Water-absorption Scaffold Release Polymer testing Polymer degradation Polylactide Polyester Poly(lactide-co-glycolide) (plga) Plga Performance In vitro validation Fibrous scaffolds Erosion Degradation behavior Biocomposites Biocompatibility Bastides
Resum: Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.
Àrees temàtiques: Química Polymers and plastics Polymer science Organic chemistry Odontología Medicina ii Medicina i Materials science, characterization & testing Materiais Interdisciplinar Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Astronomia / física
ISSN: 01429418
Adreça de correu electrònic de l'autor: angels.serra@urv.cat
Identificador de l'autor: 0000-0003-1387-0358
Pàgina final: 267
Data d'alta del registre: 2024-09-07
Volum de revista: 66
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0142941817315647
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Polymer Testing. 66 256-267
Referència de l'ítem segons les normes APA: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus (2018). In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case. Polymer Testing, 66(), 256-267. DOI: 10.1016/j.polymertesting.2018.01.027
DOI de l'article: 10.1016/j.polymertesting.2018.01.027
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2018
Pàgina inicial: 256
Tipus de publicació: Journal Publications