Articles producció científica> Química Analítica i Química Orgànica

Structure-Based Design of Potent Tumor-Associated Antigens: Modulation of Peptide Presentation by Single-Atom O/S or O/Se Substitutions at the Glycosidic Linkage

  • Dades identificatives

    Identificador: imarina:5873698
    Handle: https://hdl.handle.net/20.500.11797/imarina5873698
    Autors:
    Companon, IsmaelGuerreiro, AnaMangini, VincenzoCastro-Lopez, JorgeEscudero-Casao, MargaritaAvenoza, AlbertoBusto, Jesus HCastillon, SergioJimenez-Barbero, JesusAsensio, Juan LJimenez-Oses, GonzaloBoutureira, OmarPeregrina, Jesus MHurtado-Guerrero, RamonFiammengo, RobertoBernardes, Goncalo J LCorzana, Francisco
    Resum:
    GalNAc-glycopeptides derived from mucin MUC1 are an important class of tumor-associated antigens. α- O-glycosylation forces the peptide to adopt an extended conformation in solution, which is far from the structure observed in complexes with a model anti-MUC1 antibody. Herein, we propose a new strategy for designing potent antigen mimics based on modulating peptide/carbohydrate interactions by means of O → S/Se replacement at the glycosidic linkage. These minimal chemical modifications bring about two key structural changes to the glycopeptide. They increase the carbohydrate-peptide distance and change the orientation and dynamics of the glycosidic linkage. As a result, the peptide acquires a preorganized and optimal structure suited for antibody binding. Accordingly, these new glycopeptides display improved binding toward a representative anti-MUC1 antibody relative to the native antigens. To prove the potential of these glycopeptides as tumor-associated MUC1 antigen mimics, the derivative bearing the S-glycosidic linkage was conjugated to gold nanoparticles and tested as an immunogenic formulation in mice without any adjuvant, which resulted in a significant humoral immune response. Importantly, the mice antisera recognize cancer cells in biopsies of breast cancer patients with high selectivity. This finding demonstrates that the antibodies elicited against the mimetic antigen indeed recognize the naturally occurring antigen in its physiological context. Clinically, the exploitation of tumor-associated antigen mimics may contribute to the development of cancer vaccines and to the improvement of cancer diagnosis based on anti-MUC1 antibodies. The methodology presented here is of general interest for applications because it may be extended to modulate the affinity of bi

  • Altres:

    Autor segons l'article: Companon, Ismael; Guerreiro, Ana; Mangini, Vincenzo; Castro-Lopez, Jorge; Escudero-Casao, Margarita; Avenoza, Alberto; Busto, Jesus H; Castillon, Sergio; Jimenez-Barbero, Jesus; Asensio, Juan L; Jimenez-Oses, Gonzalo; Boutureira, Omar; Peregrina, Jesus M; Hurtado-Guerrero, Ramon; Fiammengo, Roberto; Bernardes, Goncalo J L; Corzana, Francisco
    Departament: Química Analítica i Química Orgànica
    Autor/s de la URV: Boutureira Martín, Omar / Castillón Miranda, Sergio
    Paraules clau: Vaccines Tn-antigen Sulfur Selenium Oxygen O-glycosylation Muc1 Molecular structure Molecular recognition Mice, inbred balb c Mice Mammary neoplasms, experimental Humans Glycosylation Glycosides Glycopeptides Female Drug design Conformations Carbohydrates Carbohydrate Cancer Breast neoplasms Antigens, neoplasm Antibodies, monoclonal Antibodies Animals
    Resum: GalNAc-glycopeptides derived from mucin MUC1 are an important class of tumor-associated antigens. α- O-glycosylation forces the peptide to adopt an extended conformation in solution, which is far from the structure observed in complexes with a model anti-MUC1 antibody. Herein, we propose a new strategy for designing potent antigen mimics based on modulating peptide/carbohydrate interactions by means of O → S/Se replacement at the glycosidic linkage. These minimal chemical modifications bring about two key structural changes to the glycopeptide. They increase the carbohydrate-peptide distance and change the orientation and dynamics of the glycosidic linkage. As a result, the peptide acquires a preorganized and optimal structure suited for antibody binding. Accordingly, these new glycopeptides display improved binding toward a representative anti-MUC1 antibody relative to the native antigens. To prove the potential of these glycopeptides as tumor-associated MUC1 antigen mimics, the derivative bearing the S-glycosidic linkage was conjugated to gold nanoparticles and tested as an immunogenic formulation in mice without any adjuvant, which resulted in a significant humoral immune response. Importantly, the mice antisera recognize cancer cells in biopsies of breast cancer patients with high selectivity. This finding demonstrates that the antibodies elicited against the mimetic antigen indeed recognize the naturally occurring antigen in its physiological context. Clinically, the exploitation of tumor-associated antigen mimics may contribute to the development of cancer vaccines and to the improvement of cancer diagnosis based on anti-MUC1 antibodies. The methodology presented here is of general interest for applications because it may be extended to modulate the affinity of biologically relevant glycopeptides toward their receptors.
    Àrees temàtiques: Química Materiais Interdisciplinar General chemistry Farmacia Engenharias iv Engenharias iii Engenharias ii Colloid and surface chemistry Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Biochemistry Astronomia / física
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00027863
    Adreça de correu electrònic de l'autor: omar.boutureira@urv.cat sergio.castillon@urv.cat
    Identificador de l'autor: 0000-0002-0768-8309 0000-0002-0690-7549
    Data d'alta del registre: 2024-11-30
    Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Journal Of The American Chemical Society. 141 (9): 4063-4072
    Referència de l'ítem segons les normes APA: Companon, Ismael; Guerreiro, Ana; Mangini, Vincenzo; Castro-Lopez, Jorge; Escudero-Casao, Margarita; Avenoza, Alberto; Busto, Jesus H; Castillon, Serg (2019). Structure-Based Design of Potent Tumor-Associated Antigens: Modulation of Peptide Presentation by Single-Atom O/S or O/Se Substitutions at the Glycosidic Linkage. Journal Of The American Chemical Society, 141(9), 4063-4072. DOI: 10.1021/jacs.8b13503
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2019
    Tipus de publicació: Journal Publications

  • Paraules clau:

    Biochemistry,Catalysis,Chemistry,Chemistry (Miscellaneous),Chemistry, Multidisciplinary,Colloid and Surface Chemistry
    Vaccines
    Tn-antigen
    Sulfur
    Selenium
    Oxygen
    O-glycosylation
    Muc1
    Molecular structure
    Molecular recognition
    Mice, inbred balb c
    Mice
    Mammary neoplasms, experimental
    Humans
    Glycosylation
    Glycosides
    Glycopeptides
    Female
    Drug design
    Conformations
    Carbohydrates
    Carbohydrate
    Cancer
    Breast neoplasms
    Antigens, neoplasm
    Antibodies, monoclonal
    Antibodies
    Animals
    Química
    Materiais
    Interdisciplinar
    General chemistry
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Colloid and surface chemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Chemistry
    Catalysis
    Biochemistry
    Astronomia / física

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