Autor segons l'article: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Rozès, Nicolas Andre Louis
Paraules clau: Yeast Saccharomyces cerevisiae Ole1 Mga2 Iron deficiency Fatty acids Aft1 saccharomyces cerevisiae ole1 mga2 iron deficiency fatty acids aft1
Resum: © 2020 Elsevier B.V. Iron is a vital micronutrient for all eukaryotes because it participates as a redox cofactor in multiple metabolic pathways, including lipid biosynthesis. In response to iron deficiency, the Saccharomyces cerevisiae iron-responsive transcription factor Aft1 accumulates in the nucleus and activates a set of genes that promote iron acquisition at the cell surface. In this study, we report that yeast cells lacking the transcription factor Mga2, which promotes the expression of the iron-dependent Δ9-fatty acid desaturase Ole1, display a defect in the activation of the iron regulon during the adaptation to iron limitation. Supplementation with exogenous unsaturated fatty acids (UFAs) or OLE1 expression rescues the iron regulon activation defect of mga2Δ cells. These observations and fatty acid measurements suggest that the mga2Δ defect in iron regulon expression is due to low UFA levels. Subcellular localization studies reveal that low UFAs cause a mislocalization of Aft1 protein to the vacuole upon iron deprivation that prevents its nuclear accumulation. These results indicate that Mga2 and Ole1 are essential to maintain the UFA levels required for Aft1-dependent activation of the iron regulon in response to iron deficiency, and directly connect the biosynthesis of fatty acids to the response to iron depletion.
Àrees temàtiques: Química Molecular biology Medicina ii Medicina i Linguística e literatura Interdisciplinar Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biophysics Biodiversidade Biochemistry & molecular biology
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1388-1981
Adreça de correu electrònic de l'autor: nicolasrozes@urv.cat
Identificador de l'autor: 0000-0001-9718-3429
Data d'alta del registre: 2023-02-22
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S1388198120300998?via%3Dihub
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1865 (8): 158707-
Referència de l'ítem segons les normes APA: Jordá T; Romero AM; Perea-García A; Rozès N; Puig S (2020). The lipid composition of yeast cells modulates the response to iron deficiency. Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids, 1865(8), 158707-. DOI: 10.1016/j.bbalip.2020.158707
DOI de l'article: 10.1016/j.bbalip.2020.158707
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2020
Tipus de publicació: Journal Publications
Repositori URV