Liver fat deposition and mitochondrial dysfunction in morbid obesity: An approach combining metabolomics with liver imaging and histology

Autor segons l'article: Calvo, Nahum; Beltran-Debon, Raul; Rodriguez-Gallego, Esther; Hernandez-Aguilera, Anna; Guirro, Maria; Marine-Casado, Roger; Milla, Lidon; Alegret, Josep M; Sabench, Fatima; del Castillo, Daniel; Vinaixa, Maria; Angel Rodriguez, Miguel; Correig, Xavier; Garcia-Alvarez, Roberto; Menendez, Javier A; Camps, Jordi; Joven, Jorge

Departament: Enginyeria Electrònica, Elèctrica i Automàtica; Medicina i Cirurgia; Bioquímica i Biotecnologia

Autor/s de la URV: Alegret Colomé, Josep Maria / Beltrán Debón, Raúl Alejandro / Camps Andreu, Jorge / CAMPS RATERA, JOSEP / Correig Blanchar, Francesc Xavier / Del Castillo Déjardin, Daniel / Joven Maried, Jorge / Rodríguez Chacón, Matilde / Rodríguez Gallego, Esther / Sabench Pereferrer, Fàtima / Vinaixa Crevillent, Maria

Paraules clau: Treatment outcome; Time factors; Spain; Severity of illness index; Reproducibility of results; Remission induction; Proton magnetic resonance spectroscopy; Prospective studies; Predictive value of tests; Obesity, morbid; Non-alcoholic steatohepatitis; Non-alcoholic fatty liver disease; Morbid obesity; Mitochondrial function; Mitochondrial diseases; Mitochondria, liver; Middle aged; Metabolomics; Male; Magnetic resonance spectroscopy; Magnetic resonance imaging; Liver; Lipids; Humans; Female; Fatty liver disease; Cross-sectional studies; Biopsy; Biomarkers; Bariatric surgery; Adult; non-alcoholic fatty liver disease; morbid obesity; mitochondrial function; metabolomics; magnetic resonance spectroscopy; magnetic resonance imaging; lipids; fatty liver disease

Resum: © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved. AIM: To explore the usefulness of magnetic resonance imaging (MRI) and spectroscopy (MRS) for assessment of non-alcoholic fat liver disease (NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations. RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, which excluded the presence of non-alcoholic steatohepatitis (NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak (0.9 ppm), the-CH2-peak (1.3 ppm, mostly triglyceride) and the-CH=CH-peak (2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may help to explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients. CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose.

Àrees temàtiques: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Planejamento urbano e regional / demografia; Odontología; Nutrição; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Interdisciplinar; Gastroenterology & hepatology; Gastroenterology; Farmacia; Engenharias iv; Engenharias ii; Enfermagem; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Biotecnología; Biodiversidade

Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 10079327

Adreça de correu electrònic de l'autor: maria.vinaixa@urv.cat; jorge.camps@urv.cat; danieldel.castillo@urv.cat; matilde.rodriguez@urv.cat; maria.vinaixa@urv.cat; jorge.joven@urv.cat; josepmaria.alegret@urv.cat; fatima.sabench@urv.cat; esther.rodriguez@urv.cat; xavier.correig@urv.cat; raul.beltran@urv.cat

Data d'alta del registre: 2025-02-19

Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion

Enllaç font original: https://www.wjgnet.com/1007-9327/full/v21/i24/7529.htm

URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/

Referència a l'article segons font original: World Journal Of Gastroenterology. 21 (24): 7529-7544

Referència de l'ítem segons les normes APA: Calvo, Nahum; Beltran-Debon, Raul; Rodriguez-Gallego, Esther; Hernandez-Aguilera, Anna; Guirro, Maria; Marine-Casado, Roger; Milla, Lidon; Alegret, Jo (2015). Liver fat deposition and mitochondrial dysfunction in morbid obesity: An approach combining metabolomics with liver imaging and histology. World Journal Of Gastroenterology, 21(24), 7529-7544. DOI: 10.3748/wjg.v21.i24.7529

DOI de l'article: 10.3748/wjg.v21.i24.7529

Entitat: Universitat Rovira i Virgili

Any de publicació de la revista: 2015

Tipus de publicació: Journal Publications