Articles producció científica> Ciències Mèdiques Bàsiques

Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction

  • Dades identificatives

    Identificador: imarina:6388748
    Handle: https://hdl.handle.net/20.500.11797/imarina6388748
    Autors:
    Nadal LGarcia NHurtado ESimó ATomàs MLanuza MSantafé MTomàs J
    Resum:
    © 2016 The Author(s). Background: The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons with different activities (the least active are punished) leads to the loss of roughly half of the overproduced elements and this refines connectivity and increases specificity. The neuromuscular junction is innervated by a single axon at the end of the synapse elimination process and, because of its relative simplicity, has long been used as a model for studying the general principles of synapse development. The involvement of the presynaptic muscarinic ACh autoreceptors may allow for the direct competitive interaction between nerve endings through differential activity-dependent acetylcholine release in the synaptic cleft. Then, the most active ending may directly punish the less active ones. Our previous results indicate the existence in the weakest axons on the polyinnervated neonatal NMJ of an ACh release inhibition mechanism based on mAChR coupled to protein kinase C and voltage-dependent calcium channels. We suggest that this mechanism plays a role in the elimination of redundant neonatal synapses. Results: Here we used confocal microscopy and quantitative morphological analysis to count the number of brightly fluorescent axons per endplate in P7, P9 and P15 transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice. We investigate the involvement of individual mAChR M1-, M2- and M4-subtypes in the control of axonal elimination after the Levator auris longus muscle had been exposed to agonist and antagonist in vivo. We also analysed the role of adenosine receptor subtypes (A1 and A2A) and the tropomyosin-rela

  • Altres:

    Autor segons l'article: Nadal L; Garcia N; Hurtado E; Simó A; Tomàs M; Lanuza M; Santafé M; Tomàs J
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: García Gutiérrez, Nerea / Garcia Sancho, Maria de les Neus / Hurtado Caballero, Erica / Lanuza Escolano, María Angel / NADAL MAGRIÑÀ, LAURA / Santafé Martínez, Manuel / SIMÓ OLLÉ, ANNA / Tomás Ferré, José Maria / Tomas Marginet, Marta / TOMÀS ROIG, JORDI
    Paraules clau: Weak inputs Transmitter release Serine kinases Postsynaptic maturation Postnatal maturation Neurotransmitter release Neuromuscular junction Nerve muscle junctions Motor nerve terminal Motor end-plate In-vivo Cholinergic synapses Calcium-channels C activity
    Resum: © 2016 The Author(s). Background: The development of the nervous system involves an initially exuberant production of neurons that make an excessive number of synaptic contacts. The initial overproduction of synapses promotes connectivity. Hebbian competition between axons with different activities (the least active are punished) leads to the loss of roughly half of the overproduced elements and this refines connectivity and increases specificity. The neuromuscular junction is innervated by a single axon at the end of the synapse elimination process and, because of its relative simplicity, has long been used as a model for studying the general principles of synapse development. The involvement of the presynaptic muscarinic ACh autoreceptors may allow for the direct competitive interaction between nerve endings through differential activity-dependent acetylcholine release in the synaptic cleft. Then, the most active ending may directly punish the less active ones. Our previous results indicate the existence in the weakest axons on the polyinnervated neonatal NMJ of an ACh release inhibition mechanism based on mAChR coupled to protein kinase C and voltage-dependent calcium channels. We suggest that this mechanism plays a role in the elimination of redundant neonatal synapses. Results: Here we used confocal microscopy and quantitative morphological analysis to count the number of brightly fluorescent axons per endplate in P7, P9 and P15 transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice. We investigate the involvement of individual mAChR M1-, M2- and M4-subtypes in the control of axonal elimination after the Levator auris longus muscle had been exposed to agonist and antagonist in vivo. We also analysed the role of adenosine receptor subtypes (A1 and A2A) and the tropomyosin-related kinase B receptor. The data show that postnatal axonal elimination is a regulated multireceptor mechanism that guaranteed the monoinnervation of the neuromuscular synapses. Conclusion: The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings, possibly helping to determine the winner or the lossers but, thereafter, the final elimination would occur with some autonomy and independently of postsynaptic maturation.
    Àrees temàtiques: Psicología Neurosciences Molecular biology Interdisciplinar Ciências biológicas ii Cellular and molecular neuroscience
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: marta.tomas@urv.cat nerea.garciagu@estudiants.urv.cat josepmaria.tomas@urv.cat mariaangel.lanuza@urv.cat manuel.santafe@urv.cat
    Identificador de l'autor: 0000-0002-4151-1697 0000-0002-0406-0006 0000-0003-4795-4103 0000-0002-5462-5108
    Data d'alta del registre: 2024-09-21
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Molecular Brain. 9 (1): 67-
    Referència de l'ítem segons les normes APA: Nadal L; Garcia N; Hurtado E; Simó A; Tomàs M; Lanuza M; Santafé M; Tomàs J (2016). Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction. Molecular Brain, 9(1), 67-. DOI: 10.1186/s13041-016-0248-9
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2016
    Tipus de publicació: Journal Publications

  • Paraules clau:

    Cellular and Molecular Neuroscience,Molecular Biology,Neurosciences
    Weak inputs
    Transmitter release
    Serine kinases
    Postsynaptic maturation
    Postnatal maturation
    Neurotransmitter release
    Neuromuscular junction
    Nerve muscle junctions
    Motor nerve terminal
    Motor end-plate
    In-vivo
    Cholinergic synapses
    Calcium-channels
    C activity
    Psicología
    Neurosciences
    Molecular biology
    Interdisciplinar
    Ciências biológicas ii
    Cellular and molecular neuroscience

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