Autor segons l'article: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc; Auladell, Carme; Camins, Antoni; Folch, Jaume
Departament: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
Autor/s de la URV: Folch Lopez, Jaume / Martí Zaragoza, Àlex / Martínez Micaelo, Nieves Beatriz / PATRACA FIERRO, JOSÉ IVÁN / Sureda Batlle, Francesc Xavier
Paraules clau: Tumor necrosis factor-alpha P38 mitogen-activated protein kinases P38 mapk Nitric oxide Neuroglia Mice, inbred c57bl Mice Map kinase signaling system Leptin Interleukin-1beta Interferon-gamma Inos Inflammation Glial cells Disease models, animal Cytokines Cytochrome-c Cells, cultured Cell survival Caspase-3 Apoptosis Anti-inflammatory agents Animals leptin inos glial cells cytochrome-c caspase-3
Resum: © 2016 Institute of Pharmacology, Polish Academy of Sciences Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Pharmacology & pharmacy Pharmacology Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Biotecnología
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 17341140
Adreça de correu electrònic de l'autor: neus.martinez@urv.cat alex.marti@estudiants.urv.cat jaume.folch@urv.cat francesc.sureda@urv.cat
Identificador de l'autor: 0000-0002-5051-8858 0000-0002-7968-3929
Data d'alta del registre: 2024-10-19
Versió de l'article dipositat: info:eu-repo/semantics/acceptedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273?via%3Dihub
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Pharmacological Reports. 69 (3): 409-418
Referència de l'ítem segons les normes APA: Patraca, Ivan; Martinez, Nohora; Busquets, Oriol; Marti, Aleix; Pedros, Ignacio; Beas-Zarate, Carlos; Marin, Miguel; Ettcheto, Miren; Sureda, Francesc (2017). Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition. Pharmacological Reports, 69(3), 409-418. DOI: 10.1016/j.pharep.2016.12.005
DOI de l'article: 10.1016/j.pharep.2016.12.005
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2017
Tipus de publicació: Journal Publications
Repositori URV