Autor segons l'article: López-Cano C, Gutiérrez-Carrasquilla L, Barbé F, Sánchez E, Hernández M, Martí R, Ceperuelo-Mallafre V, Dalmases M, Fernández-Veledo S, Vendrell J, Hernández C, Simó R, Lecube A
Departament: Medicina i Cirurgia Ciències Mèdiques Bàsiques
Autor/s de la URV: Ceperuelo Mallafré, Maria Victoria / Fernandez Veledo, Sonia / Vendrell Ortega, Juan José
Paraules clau: Type 2 diabetes Sleep Rhythms Night Metabolic control Hypoxia Endocrine Clock genes Circadian clock Chronodisruption Cellular adaptation Apnea
Resum: Limited reports exist on the relationships between regulation of oxygen homeostasis and circadian clock genes in type 2 diabetes. We examined whether the expression of Hypoxia-Inducible Factor-1 alpha (HIF-1 alpha) and HIF-2 alpha relates to changes in the expression of clock genes (Period homolog proteins (PER)1, PER2, PER3, Retinoid-related orphan receptor alpha (RORA), Aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL), Circadian locomotor output cycles kaput (CLOCK), and Cryptochrome proteins (CRY) 1 and CRY2) in patients with type 2 diabetes. A total of 129 subjects were evaluated in this cross-sectional study (48% with diabetes). The gene expression was measured by polymerase chain reaction. The lactate and pyruvate levels were used as surrogate of the hypoxia induced anaerobic glycolysis activity. Patients with diabetes showed an increased plasma concentration of both lactate (2102.1 +/- 688.2 vs. 1730.4 +/- 694.4 uM/L,p= 0.013) and pyruvate (61.9 +/- 25.6 vs. 50.3 +/- 23.1 uM/L,p= 0.026) in comparison to controls. However, this finding was accompanied by a blunted HIF-1 alpha expression (1.1 (0.2 to 5.0) vs. 1.7 (0.4 to 9.2) arbitrary units (AU),p <= 0.001). Patients with diabetes also showed a significant reduction of all assessed clock genes' expression. Univariate analysis showed that HIF-1 alpha and almost all clock genes were significantly and negatively correlated with HbA1c concentration. In addition, positive correlations between HIF-1 alpha and the clock genes were observed. The stepwise multivariate regression analysis showed that HbA1c and clock genes independently predicted the expression of HIF-1 alpha. Type 2 diabetes modifies the expression of HIF-1 alpha and clock genes, which correlates with the degree of metabolic control.
Àrees temàtiques: Medicine, general & internal Medicine (miscellaneous) Medicine (all)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: victoria.ceperuelo@urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
Identificador de l'autor: 0000-0002-4460-9761 0000-0003-2906-3788 0000-0002-6994-6115
Data d'alta del registre: 2024-09-07
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2077-0383/9/8/2632
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Journal Of Clinical Medicine. 9 (8): 1-10
Referència de l'ítem segons les normes APA: López-Cano C, Gutiérrez-Carrasquilla L, Barbé F, Sánchez E, Hernández M, Martí R, Ceperuelo-Mallafre V, Dalmases M, Fernández-Veledo S, Vendrell J, He (2020). Effect of Type 2 Diabetes Mellitus on the Hypoxia-Inducible Factor 1-Alpha Expression. Is There a Relationship with the Clock Genes?. Journal Of Clinical Medicine, 9(8), 1-10. DOI: 10.3390/jcm9082632
DOI de l'article: 10.3390/jcm9082632
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2020
Tipus de publicació: Journal Publications