Articles producció científica> Enginyeria Química

Characterization of river biofilm responses to the exposure with heavy metals using a novel micro fluorometer biosensor

  • Dades identificatives

    Identificador: imarina:9139026
    Autors:
    Roberta CLorenzo NELlopart JSKumar VSchuhmacher M
    Resum:
    © 2020 Elsevier B.V. River biofilms are a suitable indicator of toxic stress in aquatic ecosystems commonly exposed to various anthropogenic pollutants from industrial, domestic, and agricultural sources. Among these pollutants, heavy metals are of particular concern as they are known to interfere with various physiological processes of river biofilm, directly or indirectly related to photosynthetic performance. Nevertheless, only limited toxicological data are available on the mechanisms and toxicodynamics of heavy metals in biofilms. Pulse Amplitude Modulated (PAM) fluorometry is a rapid, non-disruptive, well-established technique to monitor toxic responses on photosynthetic performance, fluorescence-kinetics, and changes in yield in other non-photochemical processes. In this study, a new micro-PAM-sensor was tested to assess potential acute and chronic effects of heavy metals in river biofilm. Toxicity values across the three parameters considered in this study (photosynthetic yield YII, non-photochemical quenching NPQ, and basal fluorescence F0) were comparable, as determined EC50 were within one order of magnitude (EC50 ∼1−10 mg L−1). However, the stimulation of NPQ was more clearly associated with early acute effects, especially in illuminated samples, while depression of YII and F0 were more prevalent in chronic tests. These results have implications for the development of functional indicators for the biomonitoring of aquatic health, in particular for the use of river biofilm as a bioindicator of water quality. In conclusion, the approach proposed seems promising to characterize and monitor the exposure and impact of heavy metals on river periphyton communities. Furthermore, this study provides a fast, highly sensitive, inexpensive, and accurate laboratory metho
  • Altres:

    Autor segons l'article: Roberta C; Lorenzo NE; Llopart JS; Kumar V; Schuhmacher M
    Departament: Enginyeria Química
    Autor/s de la URV: Kumar, Vikas / Schuhmacher Ansuategui, Marta
    Codi de projecte: TECSPR17-1-0012
    Paraules clau: Toxicity Surface water monitoring River biofilm Photosynthesis Pam-fluorometry Impacts Heavy metal Functional recovery Fresh-water Fluvial biofilms Fluorescence Ec50 Copper Communities Chemical speciation
    Resum: © 2020 Elsevier B.V. River biofilms are a suitable indicator of toxic stress in aquatic ecosystems commonly exposed to various anthropogenic pollutants from industrial, domestic, and agricultural sources. Among these pollutants, heavy metals are of particular concern as they are known to interfere with various physiological processes of river biofilm, directly or indirectly related to photosynthetic performance. Nevertheless, only limited toxicological data are available on the mechanisms and toxicodynamics of heavy metals in biofilms. Pulse Amplitude Modulated (PAM) fluorometry is a rapid, non-disruptive, well-established technique to monitor toxic responses on photosynthetic performance, fluorescence-kinetics, and changes in yield in other non-photochemical processes. In this study, a new micro-PAM-sensor was tested to assess potential acute and chronic effects of heavy metals in river biofilm. Toxicity values across the three parameters considered in this study (photosynthetic yield YII, non-photochemical quenching NPQ, and basal fluorescence F0) were comparable, as determined EC50 were within one order of magnitude (EC50 ∼1−10 mg L−1). However, the stimulation of NPQ was more clearly associated with early acute effects, especially in illuminated samples, while depression of YII and F0 were more prevalent in chronic tests. These results have implications for the development of functional indicators for the biomonitoring of aquatic health, in particular for the use of river biofilm as a bioindicator of water quality. In conclusion, the approach proposed seems promising to characterize and monitor the exposure and impact of heavy metals on river periphyton communities. Furthermore, this study provides a fast, highly sensitive, inexpensive, and accurate laboratory method to test effects of pollutants on complex periphyton communities that can also give insights regarding the probable toxicological mechanisms of heavy metals on photosynthetic performance in the river biofilm.
    Àrees temàtiques: Zootecnia / recursos pesqueiros Toxicology Química Odontología Medicina veterinaria Medicina i Materiais Marine & freshwater biology Interdisciplinar Health, toxicology and mutagenesis Geociências General medicine Filosofía Farmacia Engenharias iii Engenharias i Educação física Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade Astronomia / física Aquatic science
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: vikas.kumar@urv.cat marta.schuhmacher@urv.cat
    Identificador de l'autor: 0000-0002-9795-5967 0000-0003-4381-2490
    Data d'alta del registre: 2024-07-27
    Versió de l'article dipositat: info:eu-repo/semantics/submittedVersion
    Enllaç font original: https://www.sciencedirect.com/science/article/abs/pii/S0166445X20304811
    Programa de finançament: TECNIOspring PLUS
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Aquatic Toxicology. 231 (105732): 105732-
    Referència de l'ítem segons les normes APA: Roberta C; Lorenzo NE; Llopart JS; Kumar V; Schuhmacher M (2021). Characterization of river biofilm responses to the exposure with heavy metals using a novel micro fluorometer biosensor. Aquatic Toxicology, 231(105732), 105732-. DOI: 10.1016/j.aquatox.2020.105732
    Acrònim: TOXMIC Toxicant monitoring chamber for microcontaminants
    DOI de l'article: 10.1016/j.aquatox.2020.105732
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2021
    Acció del programa de finançament: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 712949 and the Government of Catalonia's Agency for Business Competitiveness(ACCIÓ)
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Aquatic Science,Health, Toxicology and Mutagenesis,Marine & Freshwater Biology,Toxicology
    Toxicity
    Surface water monitoring
    River biofilm
    Photosynthesis
    Pam-fluorometry
    Impacts
    Heavy metal
    Functional recovery
    Fresh-water
    Fluvial biofilms
    Fluorescence
    Ec50
    Copper
    Communities
    Chemical speciation
    Zootecnia / recursos pesqueiros
    Toxicology
    Química
    Odontología
    Medicina veterinaria
    Medicina i
    Materiais
    Marine & freshwater biology
    Interdisciplinar
    Health, toxicology and mutagenesis
    Geociências
    General medicine
    Filosofía
    Farmacia
    Engenharias iii
    Engenharias i
    Educação física
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Biotecnología
    Biodiversidade
    Astronomia / física
    Aquatic science
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