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Scedosporium spp. from Clinical Setting in Argentina, with the Proposal of the New Pathogenic Species Scedosporium americanum

  • Dades identificatives

    Identificador: imarina:9187098
    Autors:
    Abrantes, Ruben A.Refojo, NicolasHevia, Alejandra I.Fernandez, JulianIsla, GuillerminaCordoba, SusanaDavalos, Maria F.Lubovich, SilvinaMaldonado, IvanaDavel, Graciela O.Stchigel, Alberto M.
    Resum:
    Species of the genus Scedosporium (family Microascaceae, phylum Ascomycota) are responsible for a wide range of opportunistic human infections, and have a low susceptibility to most antifungal drugs. It is well known that the pattern of Scedosporium species distribution varies according to geographic region. To assess the diversity of Scedosporium species in Argentina involved in human infections, we carried out a retrospective study reviewing 49 strains from clinical samples sent for diagnosis to the National Clinical Mycology Reference Laboratory between 1985 and 2019. Then, a phenotypic characterization, a phylogenetic study and and in vitro susceptibility test to antifungals were carried out. An analysis of combined nucleotide sequences dataset of the internal transcribed spacer of the ribosomal DNA (ITS) and of a fragment of the beta-tubulin gene (BT2) demonstrated that 92% of the strains belonged to the species S. boydii, S. apiospermum and S. angustum, all them pertaining to S. apiospermum species complex. However, two strains (4%) were identified as S. aurantiacum, a species never reported in clinical settings in the Americas'. Surprisingly, one of them displayed a polycytella-like conidiogenesis, up to date only reported for S. apiospermum. In addition, the strain DMic 165285 was phylogenetically located far away from the rest of the species, so is proposed as the novel species Scedosporium americanum. On the other hand, from all seven antifungals tested, voriconazole and posaconazole were the most active drugs against Scedosporium spp.
  • Altres:

    Autor segons l'article: Abrantes, Ruben A.; Refojo, Nicolas; Hevia, Alejandra I.; Fernandez, Julian; Isla, Guillermina; Cordoba, Susana; Davalos, Maria F.; Lubovich, Silvina; Maldonado, Ivana; Davel, Graciela O.; Stchigel, Alberto M.;
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: Stchigel Glikman, Alberto Miguel
    Paraules clau: Scedosporium Phylogeny Microascales Dna sequencing Antifungal susceptibility
    Resum: Species of the genus Scedosporium (family Microascaceae, phylum Ascomycota) are responsible for a wide range of opportunistic human infections, and have a low susceptibility to most antifungal drugs. It is well known that the pattern of Scedosporium species distribution varies according to geographic region. To assess the diversity of Scedosporium species in Argentina involved in human infections, we carried out a retrospective study reviewing 49 strains from clinical samples sent for diagnosis to the National Clinical Mycology Reference Laboratory between 1985 and 2019. Then, a phenotypic characterization, a phylogenetic study and and in vitro susceptibility test to antifungals were carried out. An analysis of combined nucleotide sequences dataset of the internal transcribed spacer of the ribosomal DNA (ITS) and of a fragment of the beta-tubulin gene (BT2) demonstrated that 92% of the strains belonged to the species S. boydii, S. apiospermum and S. angustum, all them pertaining to S. apiospermum species complex. However, two strains (4%) were identified as S. aurantiacum, a species never reported in clinical settings in the Americas'. Surprisingly, one of them displayed a polycytella-like conidiogenesis, up to date only reported for S. apiospermum. In addition, the strain DMic 165285 was phylogenetically located far away from the rest of the species, so is proposed as the novel species Scedosporium americanum. On the other hand, from all seven antifungals tested, voriconazole and posaconazole were the most active drugs against Scedosporium spp.
    Àrees temàtiques: Plant science Mycology Microbiology (medical) Microbiology Farmacia Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: albertomiguel.stchigel@urv.cat
    Identificador de l'autor: 0000-0003-3987-7996
    Data d'alta del registre: 2024-07-27
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2309-608X/7/3/160
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: J Fungi (Basel). 7 (3): 1-16
    Referència de l'ítem segons les normes APA: Abrantes, Ruben A.; Refojo, Nicolas; Hevia, Alejandra I.; Fernandez, Julian; Isla, Guillermina; Cordoba, Susana; Davalos, Maria F.; Lubovich, Silvina; (2021). Scedosporium spp. from Clinical Setting in Argentina, with the Proposal of the New Pathogenic Species Scedosporium americanum. J Fungi (Basel), 7(3), 1-16. DOI: 10.3390/jof7030160
    DOI de l'article: 10.3390/jof7030160
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2021
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Ecology, Evolution, Behavior and Systematics,Microbiology,Microbiology (Medical),Mycology,Plant Science
    Scedosporium
    Phylogeny
    Microascales
    Dna sequencing
    Antifungal susceptibility
    Plant science
    Mycology
    Microbiology (medical)
    Microbiology
    Farmacia
    Ecology, evolution, behavior and systematics
    Ciências biológicas iii
    Ciências biológicas ii
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