Autor segons l'article: Abrantes, Ruben A.; Refojo, Nicolas; Hevia, Alejandra I.; Fernandez, Julian; Isla, Guillermina; Cordoba, Susana; Davalos, Maria F.; Lubovich, Silvina; Maldonado, Ivana; Davel, Graciela O.; Stchigel, Alberto M.;
Departament: Ciències Mèdiques Bàsiques
Autor/s de la URV: Stchigel Glikman, Alberto Miguel
Paraules clau: Scedosporium Phylogeny Microascales Dna sequencing Antifungal susceptibility
Resum: Species of the genus Scedosporium (family Microascaceae, phylum Ascomycota) are responsible for a wide range of opportunistic human infections, and have a low susceptibility to most antifungal drugs. It is well known that the pattern of Scedosporium species distribution varies according to geographic region. To assess the diversity of Scedosporium species in Argentina involved in human infections, we carried out a retrospective study reviewing 49 strains from clinical samples sent for diagnosis to the National Clinical Mycology Reference Laboratory between 1985 and 2019. Then, a phenotypic characterization, a phylogenetic study and and in vitro susceptibility test to antifungals were carried out. An analysis of combined nucleotide sequences dataset of the internal transcribed spacer of the ribosomal DNA (ITS) and of a fragment of the beta-tubulin gene (BT2) demonstrated that 92% of the strains belonged to the species S. boydii, S. apiospermum and S. angustum, all them pertaining to S. apiospermum species complex. However, two strains (4%) were identified as S. aurantiacum, a species never reported in clinical settings in the Americas'. Surprisingly, one of them displayed a polycytella-like conidiogenesis, up to date only reported for S. apiospermum. In addition, the strain DMic 165285 was phylogenetically located far away from the rest of the species, so is proposed as the novel species Scedosporium americanum. On the other hand, from all seven antifungals tested, voriconazole and posaconazole were the most active drugs against Scedosporium spp.
Àrees temàtiques: Plant science Mycology Microbiology (medical) Microbiology Farmacia Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: albertomiguel.stchigel@urv.cat
Identificador de l'autor: 0000-0003-3987-7996
Data d'alta del registre: 2023-02-19
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2309-608X/7/3/160
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: J Fungi (Basel). 7 (3): 1-16
Referència de l'ítem segons les normes APA: Abrantes, Ruben A.; Refojo, Nicolas; Hevia, Alejandra I.; Fernandez, Julian; Isla, Guillermina; Cordoba, Susana; Davalos, Maria F.; Lubovich, Silvina; (2021). Scedosporium spp. from Clinical Setting in Argentina, with the Proposal of the New Pathogenic Species Scedosporium americanum. J Fungi (Basel), 7(3), 1-16. DOI: 10.3390/jof7030160
DOI de l'article: 10.3390/jof7030160
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2021
Tipus de publicació: Journal Publications
Repositori URV