Articles producció científica> Bioquímica i Biotecnologia

Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1

  • Dades identificatives

    Identificador: imarina:9220654
    Autors:
    López-Fernández-Sobrino, RaúlSoliz-Rueda, Jorge R.Ávila-Román, JavierArola-Arnal, AnnaSuárez, ManuelMuguerza, BegoñaBravo, Francisca Isabel
    Resum:
    The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) were orally administered water or WLPW (125 mg/kg bw). Posteriorly, both groups were intraperitoneally administered saline, N?-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, indomethacin, a prostacyclin synthesis inhibitor, or sirtinol, an inhibitor of sirtuins. Blood pressure (BP) was recorded before and 6 h after WLPW administration. In an additional experiment, SHR were administered water or WLPW and endothelial expressions of eNos, Sirt1, Nox4, and Et1 were determined. The BP-lowering properties of WLPW were abolished by L-NAME and partially reduced by indomethacin, demonstrating that WLPW antihypertensive effect was mediated by changes in NO availability, although prostacyclin also contributed to this activity. Moreover, BP-lowering effect was reduced by sirtinol, indicating that WLPW decreased BP in a SIRT1-dependent manner. Furthermore, WLPW upregulated eNos and Sirt1 and downregulated Nox4 and Et1 endothelial gene expression. These results evidence the vasoprotective effect of WLPW and show that its antihypertensive effect in SHR is endothelium dependent and mediated by SIRT1.
  • Altres:

    Autor segons l'article: López-Fernández-Sobrino, Raúl; Soliz-Rueda, Jorge R.; Ávila-Román, Javier; Arola-Arnal, Anna; Suárez, Manuel; Muguerza, Begoña; Bravo, Francisca Isabel
    Departament: Bioquímica i Biotecnologia
    Autor/s de la URV: Alkhoury, Nadine / Arola Arnal, Anna / Ávila Román, Francisco Javier / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña / Soliz Rueda, Jorge Ricardo / Suárez Recio, Manuel
    Paraules clau: Spontaneously hypertensive rats Nitric-oxide Nadph oxidase Endothelin-1 Endothelial nitric oxide synthase Endothelial function spontaneously hypertensive rats prostacyclin polyphenols oxidative stress nadph oxidase hypertensive-rats extract endothelin-1 endothelial nitric oxide synthase decrease
    Resum: <jats:p>The antihypertensive effect of wine lees powder (WLPW) from a Cabernet grape variety was related to its high content in flavanols and anthocyanins compounds. This study investigates the involvement of endothelial-derived factors and SIRT1 in its bioactivity. Spontaneously hypertensive rats (SHR) were orally administered water or WLPW (125 mg/kg bw). Posteriorly, both groups were intraperitoneally administered saline, N?-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthesis inhibitor, indomethacin, a prostacyclin synthesis inhibitor, or sirtinol, an inhibitor of sirtuins. Blood pressure (BP) was recorded before and 6 h after WLPW administration. In an additional experiment, SHR were administered water or WLPW and endothelial expressions of eNos, Sirt1, Nox4, and Et1 were determined. The BP-lowering properties of WLPW were abolished by L-NAME and partially reduced by indomethacin, demonstrating that WLPW antihypertensive effect was mediated by changes in NO availability, although prostacyclin also contributed to this activity. Moreover, BP-lowering effect was reduced by sirtinol, indicating that WLPW decreased BP in a SIRT1-dependent manner. Furthermore, WLPW upregulated eNos and Sirt1 and downregulated Nox4 and Et1 endothelial gene expression. These results evidence the vasoprotective effect of WLPW and show that its antihypertensive effect in SHR is endothelium dependent and mediated by SIRT1.</jats:p>
    Àrees temàtiques: Química Physiology Molecular biology Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Engenharias ii Clinical biochemistry Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Cell biology Biotecnología Biodiversidade Biochemistry & molecular biology Biochemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: franciscaisabel.bravo@urv.cat franciscojavier.avila@urv.cat jorgericardo.solizr@urv.cat jorgericardo.solizr@urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat nadine.alkhoury@estudiants.urv.cat manuel.suarez@urv.cat anna.arola@urv.cat begona.muguerza@urv.cat
    Identificador de l'autor: 0000-0002-6468-3088 0000-0001-9766-8178 0000-0003-0122-8253 0000-0001-6529-1345 0000-0001-7384-8588
    Data d'alta del registre: 2024-07-27
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://www.mdpi.com/2076-3921/10/7/1073
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Antioxidants. 10 (7): 1073-
    Referència de l'ítem segons les normes APA: López-Fernández-Sobrino, Raúl; Soliz-Rueda, Jorge R.; Ávila-Román, Javier; Arola-Arnal, Anna; Suárez, Manuel; Muguerza, Begoña; Bravo, Francisca Isabe (2021). Blood Pressure-Lowering Effect of Wine Lees Phenolic Compounds Is Mediated by Endothelial-Derived Factors: Role of Sirtuin 1. Antioxidants, 10(7), 1073-. DOI: 10.3390/antiox10071073
    DOI de l'article: 10.3390/antiox10071073
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2021
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Biochemistry,Biochemistry & Molecular Biology,Cell Biology,Chemistry, Medicinal,Clinical Biochemistry,Food Science,Food Science & Technology,Molecular Biology,Physiology
    Spontaneously hypertensive rats
    Nitric-oxide
    Nadph oxidase
    Endothelin-1
    Endothelial nitric oxide synthase
    Endothelial function
    spontaneously hypertensive rats
    prostacyclin
    polyphenols
    oxidative stress
    nadph oxidase
    hypertensive-rats
    extract
    endothelin-1
    endothelial nitric oxide synthase
    decrease
    Química
    Physiology
    Molecular biology
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, medicinal
    Cell biology
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Biochemistry
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