Autor segons l'article: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B
Departament: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/s de la URV: Prieto Simón, Beatriz
Paraules clau: Milk Mastitis Inflammation Immunohistochemistry Diagnosis Cytokines Bovine mammary-gland Bovine staphylococcus-aureus somatic-cells oxide affect secretion milk intramammary infection interferon-gamma innate immune-response inflammation immunohistochemistry etiologic agents escherichia-coli epithelial-cells diagnosis cytokines bovine
Resum: In naturally occurring bovine mastitis, effects of infection depend on the host inflammatory response, including the effects of secreted cytokines. Knowledge about the inflammatory and regulatory cytokines in milk cells of free-stall barn dairy cows and in naturally occurring mastitis is lacking as most studies focus on induced mastitis. Hereby, the aim of the study was to determine inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The following examinations of milk samples were performed: differential counting of somatic cells (SCC), bacteriological examination, and immunocytochemical analysis. Mean SCC increased in subclinical and clinical mastitis cases. The number of pathogenic mastitis-causing bacteria on plates increased in subclinical mastitis cases but decreased in clinical mastitis. The inflammatory and regulatory markers in the milk cells of healthy cows showed the highest mean cell numbers (%). In mastitis cases, immunoreactivity was more pronounced for IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ. Data about subclinical and clinical mastitis demonstrate inflammatory responses to intramammary infection driven by IL-1α, IL-4, and IL-17A. Moreover, the host defense response in mastitis is characterized by continuation or resolution of initial inflammation. IL-12 and INF-γ immunoreactivity was recognized to differ mastitis cases from the relative health status.
Àrees temàtiques: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: beatriz.prieto-simon@urv.cat
Identificador de l'autor: 0000-0001-8016-1565
Data d'alta del registre: 2023-02-19
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.mdpi.com/2076-0817/11/3/372
URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Pathogens. 11 (3):
Referència de l'ítem segons les normes APA: Vitenberga-Verza Z; Pilmane M; Šerstnova K; Melderis I; Gontar Ł; Kochański M; Drutowska A; Maróti G; Prieto-Simón B (2022). Identification of Inflammatory and Regulatory Cytokines IL-1α-, IL-4-, IL-6-, IL-12-, IL-13-, IL-17A-, TNF-α-, and IFN-γ-Producing Cells in the Milk of Dairy Cows with Subclinical and Clinical Mastitis. Pathogens, 11(3), -. DOI: 10.3390/pathogens11030372
DOI de l'article: 10.3390/pathogens11030372
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV