Autor segons l'article: Shkembi, Xhensila; Botero, Mary Luz; Skouridou, Vasso; Jauset-Rubio, Miriam; Svobodova, Marketa; Ballester, Pablo; Bashammakh, Abdulaziz S; El-Shahawi, Mohammad S; Alyoubi, Abdulrahman O; Sullivan, Ciara K O
Departament: Enginyeria Química
Autor/s de la URV: BOTERO GALLEGO, MARY LUZ / Jauset Rubio, Miriam / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
Paraules clau: Testosterone congeners Testosterone Selex Performance-enhancing substances Nandrolone Metal nanoparticles Humans Gold nanoparticle assay Gold Doping in sports Doping Colorimetry Aptamers Androgenic steroids Anabolic agents Adolescent testosterone selex selection nortestosterone nandrolone metabolites human urine gold nanoparticle assay elisa doping assay antibodies 19-nortestosterone
Resum: The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.Copyright © 2022. Published by Elsevier Inc.
Àrees temàtiques: Química Odontología Molecular biology Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Engenharias iv Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Chemistry, analytical Cell biology Biotecnología Biophysics Biochemistry & molecular biology Biochemistry Biochemical research methods Astronomia / física
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: vasoula.skouridou@urv.cat miriam.jauset@urv.cat xhensila.shkembi@estudiants.urv.cat
Identificador de l'autor: 0000-0002-9712-5429 0000-0002-9943-6132
Data d'alta del registre: 2024-10-12
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.sciencedirect.com/science/article/pii/S0003269722003979
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
Referència a l'article segons font original: Analytical Biochemistry. 658 114937-114937
Referència de l'ítem segons les normes APA: Shkembi, Xhensila; Botero, Mary Luz; Skouridou, Vasso; Jauset-Rubio, Miriam; Svobodova, Marketa; Ballester, Pablo; Bashammakh, Abdulaziz S; El-Shahawi (2022). Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids. Analytical Biochemistry, 658(), 114937-114937. DOI: 10.1016/j.ab.2022.114937
DOI de l'article: 10.1016/j.ab.2022.114937
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2022
Tipus de publicació: Journal Publications
Repositori URV