Articles producció científica> Ciències Mèdiques Bàsiques

Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants

  • Dades identificatives

    Identificador: imarina:9292125
  • Autors:

    Gutiérrez-Martín, D
    Marquès, M
    Pons-Escoda, A
    Vidal, N
    Bruna, J
    Restrepo-Montes, E
    López-Serna, R
    García-Sayago, F
    Majos, C
    Gago-Ferrero, P
    Gil-Solsona, R
  • Altres:

    Autor segons l'article: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; Gil-Solsona, R
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: García Sayago, Francisco / Marquès Bueno, Montserrat
    Paraules clau: Suspect screening Non-target screening Lc-hrms Human tissue Human biomonitoring Exposome Bead beating suspect screening non-target screening lc-hrms human biomonitoring bead beating
    Resum: Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
    Àrees temàtiques: Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medical laboratory technology Interdisciplinar Engenharias iii Engenharias ii Clinical biochemistry Ciências biológicas iii Ciências ambientais Ciência de alimentos
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: francisco.garcia@urv.cat montserrat.marques@urv.cat
    Identificador de l'autor: 0000-0003-0594-8370 0000-0001-6302-8578
    Data d'alta del registre: 2024-08-03
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://methods-x.com/article/S2215-0161(23)00072-9/fulltext
    URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Methodsx. 10 (102069):
    Referència de l'ítem segons les normes APA: Gutiérrez-Martín, D; Marquès, M; Pons-Escoda, A; Vidal, N; Bruna, J; Restrepo-Montes, E; López-Serna, R; García-Sayago, F; Majos, C; Gago-Ferrero, P; (2023). Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants. Methodsx, 10(102069), -. DOI: 10.1016/j.mex.2023.102069
    DOI de l'article: 10.1016/j.mex.2023.102069
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Clinical Biochemistry,Medical Laboratory Technology,Multidisciplinary Sciences
    Suspect screening
    Non-target screening
    Lc-hrms
    Human tissue
    Human biomonitoring
    Exposome
    Bead beating
    suspect screening
    non-target screening
    lc-hrms
    human biomonitoring
    bead beating
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medical laboratory technology
    Interdisciplinar
    Engenharias iii
    Engenharias ii
    Clinical biochemistry
    Ciências biológicas iii
    Ciências ambientais
    Ciência de alimentos
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