Articles producció científica> Ciències Mèdiques Bàsiques

Comprehensive profiling and semi-quantification of exogenous chemicals in human urine using HRMS-based strategies

  • Dades identificatives

    Identificador: imarina:9331785
    Autors:
    Gutiérrez-Martín DRestrepo-Montes EGolovko OLópez-Serna RAalizadeh RThomaidis NSMarquès MGago-Ferrero PGil-Solsona R
    Resum:
    Chemicals infiltrate our daily experiences through multiple exposure pathways. Human biomonitoring (HBM) is routinely used to comprehensively understand these chemical interactions. Historically, HBM depended on targeted screening methods limited to a relatively small set of chemicals with triple quadrupole instruments typically. However, recent advances in high-resolution mass spectrometry (HRMS) have facilitated the use of broad-scope target, suspect, and non-target strategies, enhancing chemical exposome characterization within acceptable detection limits. Despite these advancements, establishing robust and efficient sample treatment protocols is still essential for trustworthy broad-range chemical analysis. This study sought to validate a methodology leveraging HRMS-based strategies for accurate profiling of exogenous chemicals and related metabolites in urine samples. We evaluated five extraction protocols, each encompassing various chemical classes, such as pharmaceuticals, plastic additives, personal care products, and pesticides, in terms of their extraction recoveries, linearity, matrix effect, sensitivity, and reproducibility. The most effective protocol was extensively validated and subsequently applied to 10 real human urine samples using wide-scope target analysis encompassing over 2000 chemicals. We successfully identified and semi-quantified a total of 36 chemicals using an ionization efficiency-based model, affirming the methodology's robust performance. Notably, our results dismissed the need for a deconjugation step, a typically labor-intensive and time-consuming process.© 2023. The Author(s).
  • Altres:

    Autor segons l'article: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R
    Departament: Ciències Mèdiques Bàsiques
    Autor/s de la URV: Marquès Bueno, Montserrat
    Paraules clau: Non-target Method validation Human biomonitoring (hbm) High-resolution mass spectrometry (hrms) Glucuronides Deconjugation
    Resum: Chemicals infiltrate our daily experiences through multiple exposure pathways. Human biomonitoring (HBM) is routinely used to comprehensively understand these chemical interactions. Historically, HBM depended on targeted screening methods limited to a relatively small set of chemicals with triple quadrupole instruments typically. However, recent advances in high-resolution mass spectrometry (HRMS) have facilitated the use of broad-scope target, suspect, and non-target strategies, enhancing chemical exposome characterization within acceptable detection limits. Despite these advancements, establishing robust and efficient sample treatment protocols is still essential for trustworthy broad-range chemical analysis. This study sought to validate a methodology leveraging HRMS-based strategies for accurate profiling of exogenous chemicals and related metabolites in urine samples. We evaluated five extraction protocols, each encompassing various chemical classes, such as pharmaceuticals, plastic additives, personal care products, and pesticides, in terms of their extraction recoveries, linearity, matrix effect, sensitivity, and reproducibility. The most effective protocol was extensively validated and subsequently applied to 10 real human urine samples using wide-scope target analysis encompassing over 2000 chemicals. We successfully identified and semi-quantified a total of 36 chemicals using an ionization efficiency-based model, affirming the methodology's robust performance. Notably, our results dismissed the need for a deconjugation step, a typically labor-intensive and time-consuming process.© 2023. The Author(s).
    Àrees temàtiques: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Biochemical research methods Astronomia / física Analytical chemistry
    Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
    Adreça de correu electrònic de l'autor: montserrat.marques@urv.cat
    Identificador de l'autor: 0000-0001-6302-8578
    Data d'alta del registre: 2024-07-20
    Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
    Enllaç font original: https://link.springer.com/article/10.1007/s00216-023-04998-9#citeas
    URL Document de llicència: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referència a l'article segons font original: Analytical And Bioanalytical Chemistry. 415 (29-30): 7297-
    Referència de l'ítem segons les normes APA: Gutiérrez-Martín D; Restrepo-Montes E; Golovko O; López-Serna R; Aalizadeh R; Thomaidis NS; Marquès M; Gago-Ferrero P; Gil-Solsona R (2023). Comprehensive profiling and semi-quantification of exogenous chemicals in human urine using HRMS-based strategies. Analytical And Bioanalytical Chemistry, 415(29-30), 7297-. DOI: 10.1007/s00216-023-04998-9
    DOI de l'article: 10.1007/s00216-023-04998-9
    Entitat: Universitat Rovira i Virgili
    Any de publicació de la revista: 2023
    Tipus de publicació: Journal Publications
  • Paraules clau:

    Analytical Chemistry,Biochemical Research Methods,Biochemistry,Chemistry, Analytical
    Non-target
    Method validation
    Human biomonitoring (hbm)
    High-resolution mass spectrometry (hrms)
    Glucuronides
    Deconjugation
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Educação física
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Astronomia / física
    Analytical chemistry
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