Autor segons l'article: Ghiaci, Payam; Jouhten, Paula; Martyushenko, Nikolay; Roca-Mesa, Helena; Vazquez, Jennifer; Konstantinidis, Dimitrios; Stenberg, Simon; Andrejev, Sergej; Grkovska, Kristina; Mas, Albert; Beltran, Gemma; Almaas, Eivind; Patil, Kiran R; Warringer, Jonas
Departament: Bioquímica i Biotecnologia
Autor/s de la URV: Beltran Casellas, Gemma / Mas Baron, Alberto
Paraules clau: Adaptation; Aneuploidy; Beneficial mutations; Clonal evolution; Diminishing returns; Directed molecular evolution; Dynamics; Epistasis; Evolutionary engineering; Experimental evolution; Fermentatio; Fermentation; Kudriavzevi; Metabolism; Phenotype; Population; Saccharomyces cerevisiae; Saccharomyces-cerevisiae; Strain; Wine; Yeast
Resum: Adaptive Laboratory Evolution (ALE) of microorganisms can improve the efficiency of sustainable industrial processes important to the global economy. However, stochasticity and genetic background effects often lead to suboptimal outcomes during laboratory evolution. Here we report an ALE platform to circumvent these shortcomings through parallelized clonal evolution at an unprecedented scale. Using this platform, we evolved 10(4) yeast populations in parallel from many strains for eight desired wine fermentation-related traits. Expansions of both ALE replicates and lineage numbers broadened the evolutionary search spectrum leading to improved wine yeasts unencumbered by unwanted side effects. At the genomic level, evolutionary gains in metabolic characteristics often coincided with distinct chromosome amplifications and the emergence of side-effect syndromes that were characteristic of each selection niche. Several high-performing ALE strains exhibited desired wine fermentation kinetics when tested in larger liquid cultures, supporting their suitability for application. More broadly, our high-throughput ALE platform opens opportunities for rapid optimization of microbes which otherwise could take many years to accomplish.
Àrees temàtiques: Agricultural and biological sciences (all); Agricultural and biological sciences (miscellaneous); Applied mathematics; Biochemistry & molecular biology; Biochemistry, genetics and molecular biology (all); Biochemistry, genetics and molecular biology (miscellaneous); Biotecnología; Ciências biológicas ii; Computational theory and mathematics; General agricultural and biological sciences; General biochemistry,genetics and molecular biology; General immunology and microbiology; General medicine; Immunology and microbiology (all); Immunology and microbiology (miscellaneous); Informati; Information systems; Medicine (miscellaneous)
Accès a la llicència d'ús: https://creativecommons.org/licenses/by/3.0/es/
Adreça de correu electrònic de l'autor: albert.mas@urv.cat; gemma.beltran@urv.cat
Data d'alta del registre: 2025-01-28
Versió de l'article dipositat: info:eu-repo/semantics/publishedVersion
Enllaç font original: https://www.embopress.org/doi/full/10.1038/s44320-024-00059-0
Referència a l'article segons font original: Molecular Systems Biology. 20 (10): 1109-1133
Referència de l'ítem segons les normes APA: Ghiaci, Payam; Jouhten, Paula; Martyushenko, Nikolay; Roca-Mesa, Helena; Vazquez, Jennifer; Konstantinidis, Dimitrios; Stenberg, Simon; Andrejev, Serg (2024). Highly parallelized laboratory evolution of wine yeasts for enhanced metabolic phenotypes. Molecular Systems Biology, 20(10), 1109-1133. DOI: 10.1038/s44320-024-00059-0
URL Document de llicència: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI de l'article: 10.1038/s44320-024-00059-0
Entitat: Universitat Rovira i Virgili
Any de publicació de la revista: 2024
Tipus de publicació: Journal Publications
Repositori URV