Author, as appears in the article.: Nicolas Rozès Ricardo Cordero-Otero Montse Poblet Gema López-Martínez Anna Borrull
Department: Bioquímica i Biotecnologia
URV's Author/s: ROZÈS ., NICOLAS ANDRE LOUIS CORDERO OTERO, RICARDO ROMÁN POBLET ICART, MARIA MONTSERRAT LÓPEZ MARTÍNEZ, GEMA ISABEL BORRULL RIERA, ANNA
Keywords: medium chain fatty acid decanoic acid derivative Alcohol
Abstract: Octanoic (C8) and decanoic (C10) acids are produced in hypoxic conditions by the yeast Saccharomyces cerevisiae as by-products of its metabolism and are considered fermentation inhibitors in the presence of ethanol at acidic pH. This study aims to broaden our understanding of the physiological limits between toxicity and ester production in yeast cells. To this end, the non-inhibitory concentration (NIC) and maximum inhibitory concentration (MIC) values were first established for C8 and C10 at physiological pH (5.8) without ethanol. The results showed that when these acids were added to culture medium at these values, they tended to accumulate in different cellular fractions of the yeast. While C8 was almost entirely located in the cell wall fraction, C10 was found in the endocellular fraction. Cell fatty acid detoxification was also different; while the esterification of fatty acids was more efficient in the case of C10, the peroxisome was activated regardless of which fatty acid was added. Furthermore, the study of the Pdr12 and Tpo1 transporters that evolved during the detoxification process revealed that C8 was mostly expelled by the Pdr12 carrier, which was related to higher β-oxidative damage in the presence of endocellular C10. C10 is more toxic at lower concentrations than C8. Although they are produced by yeast, the resulting intracellular medium-chain fatty acids (MCFAs) caused a level of toxicity which promoted cell death. However, MCFAs are involved in the production of beverage flavours. © 2015 John Wiley & Sons, Ltd.
Research group: Biotecnologia Enològica Biotecnologia Microbiana dels Aliments
Thematic Areas: Biochemistry and technology Bioquímica y tecnología Bioquímica i biotecnologia
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1097-0061
Author identifier: 0000-0001-9718-3429 0000-0001-5402-1278 0000-0002-9958-5736 n/a n/a
Record's date: 2015-08-05
Last page: 460
Journal volume: 32
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: http://onlinelibrary.wiley.com/doi/10.1002/yea.3071/full
Article's DOI: 10.1002/yea.3071
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
First page: 451
Publication Type: Article Artículo Article
Repositori URV