Articles producció científica> Bioquímica i Biotecnologia

Replenishment and mobilization of intracellular nitrogen pools decouples wine yeast nitrogen uptake from growth

  • Identification data

    Identifier: PC:1440
    Authors:
    Marta SanchoAlicia GutiérrezGemma BeltranJosé Manuel GuillamonJonas Warringer
    Abstract:
    Wine yeast capacity to take up nitrogen from the environment and catabolize it to support population growth, fermentation, and aroma production is critical to wine production. Under nitrogen restriction, yeast nitrogen uptake is believed to be intimately coupled to reproduction with nitrogen catabolite repression (NCR) suggested mediating this link. We provide a time- and strain-resolved view of nitrogen uptake, population growth, and NCR activity in wine yeasts. Nitrogen uptake was found to be decoupled from growth due to early assimilated nitrogen being used to replenish intracellular nitrogen pools rather than being channeled directly into reproduction. Internally accumulated nitrogen was later mobilized to support substantial population expansion after external nitrogen was depleted. On good nitrogen sources, the decoupling between nitrogen uptake and growth correlated well with relaxation of NCR repression, raising the potential that the latter may be triggered by intracellular build-up of nitrogen. No link between NCR activity and nitrogen assimilation or growth on poor nitrogen sources was found. The decoupling between nitrogen uptake and growth and its influence on NCR activity is of relevance for both wine production and our general understanding of nitrogen use.
  • Others:

    Author, as appears in the article.: Marta Sancho; Alicia Gutiérrez; Gemma Beltran; José Manuel Guillamon; Jonas Warringer
    Department: Bioquímica i Biotecnologia
    URV's Author/s: SANCHO FORNER, MARTA; Alicia Gutiérrez; BELTRAN CASELLAS, GEMMA; José Manuel Guillamon; Jonas Warringer
    Keywords: growth Wine YEAST
    Abstract: Wine yeast capacity to take up nitrogen from the environment and catabolize it to support population growth, fermentation, and aroma production is critical to wine production. Under nitrogen restriction, yeast nitrogen uptake is believed to be intimately coupled to reproduction with nitrogen catabolite repression (NCR) suggested mediating this link. We provide a time- and strain-resolved view of nitrogen uptake, population growth, and NCR activity in wine yeasts. Nitrogen uptake was found to be decoupled from growth due to early assimilated nitrogen being used to replenish intracellular nitrogen pools rather than being channeled directly into reproduction. Internally accumulated nitrogen was later mobilized to support substantial population expansion after external nitrogen was depleted. On good nitrogen sources, the decoupling between nitrogen uptake and growth correlated well with relaxation of NCR repression, raising the potential that the latter may be triggered by intracellular build-up of nitrogen. No link between NCR activity and nitrogen assimilation or growth on poor nitrogen sources was found. The decoupling between nitrogen uptake and growth and its influence on NCR activity is of relevance for both wine production and our general understanding of nitrogen use.
    Research group: Biotecnologia Enològica
    Thematic Areas: Enologia Enología Oenology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0175-7598
    Author identifier: n/a; n/a; 0000-0002-7071-205X; n/a; 0000-0001-6144-2740
    Record's date: 2016-04-14
    Last page: 3265
    Journal volume: 100
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://link.springer.com/article/10.1007/s00253-015-7273-y
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1007/s00253-015-7273-y
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    First page: 3255
    Publication Type: Article Artículo Article
  • Keywords:

    Vi -- Llevats
    growth
    Wine
    YEAST
    Enologia
    Enología
    Oenology
    0175-7598
  • Documents:

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