Author, as appears in the article.: Jordi Riu; M. Azizur R. Khana; Felismina T.C. Moreira; M. Goreti F. Sales
Department: Química Analítica i Química Orgànica
URV's Author/s: RIU RUSELL, JORDI; M. Azizur R. Khana; Felismina T.C. Moreira; M. Goreti F. Sales
Keywords: Chemical detection Electropolymerization bacteria
Abstract: This work presents a novel molecularly imprinted polymer (MIP) for the indirect detection of bacteria, by targeting an outer membrane protein on a disposable device. Protein A (PA) was selected for this purpose, as a representative protein of the outer surface of Staphylococcus aureus. The imprinted polymer was assembled directly on a film of single walled carbon nanotubes (SWCNTs), placed on screen-printed electrodes (SPEs). The MIP material was produced by electropolymerizing 3-aminophenol in the presence of the protein template (PA) using cyclic voltammetry (CV). The proteins entrapped at the polymeric backbone were digested by the action of proteolytic activity of proteinase K and then washed away to create vacant sites. The performance of the corresponding imprinted and non-imprinted electrodes was evaluated by EIS and the effect of several variables, such as monomer and template concentrations, or thickness of imprinting surface, was controlled and optimized by the number of CV cycles. The detection limit of the MIP-based sensors was 0.60 nM in MES buffer. High repeatability and good selectivity were observed in the presence of a model protein BSA. The sensor performance was also tested to check the effect of inorganic ions in tap water. The detection limit observed was 16.83 nM, with a recovery factor of 91.1 ± 6.6%. The sensor described in this work is a potential tool for screening PA on-site, due to the simplicity of fabrication, disposability, short response time, low cost, good sensitivity and selectivity.
Research group: Grup de Quimiometria, Qualimetria i Nanosensors
Thematic Areas: Chemistry Química Química
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0925-4005
Author identifier: 0000-0001-5823-9223; n/a; n/a; n/a
Record's date: 2016-05-23
Last page: 704
Journal volume: 233
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0925400516305573?via%3Dihub
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1016/j.snb.2016.04.075
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: 697
Publication Type: Post-print