Articles producció científica> Química Analítica i Química Orgànica

Plastic antibody for the electrochemical detection of bacterial surfaceproteins

  • Identification data

    Identifier: PC:1498
    Authors:
    Jordi RiuM. Azizur R. KhanaFelismina T.C. MoreiraM. Goreti F. Sales
    Abstract:
    This work presents a novel molecularly imprinted polymer (MIP) for the indirect detection of bacteria, by targeting an outer membrane protein on a disposable device. Protein A (PA) was selected for this purpose, as a representative protein of the outer surface of Staphylococcus aureus. The imprinted polymer was assembled directly on a film of single walled carbon nanotubes (SWCNTs), placed on screen-printed electrodes (SPEs). The MIP material was produced by electropolymerizing 3-aminophenol in the presence of the protein template (PA) using cyclic voltammetry (CV). The proteins entrapped at the polymeric backbone were digested by the action of proteolytic activity of proteinase K and then washed away to create vacant sites. The performance of the corresponding imprinted and non-imprinted electrodes was evaluated by EIS and the effect of several variables, such as monomer and template concentrations, or thickness of imprinting surface, was controlled and optimized by the number of CV cycles. The detection limit of the MIP-based sensors was 0.60 nM in MES buffer. High repeatability and good selectivity were observed in the presence of a model protein BSA. The sensor performance was also tested to check the effect of inorganic ions in tap water. The detection limit observed was 16.83 nM, with a recovery factor of 91.1 ± 6.6%. The sensor described in this work is a potential tool for screening PA on-site, due to the simplicity of fabrication, disposability, short response time, low cost, good sensitivity and selectivity.
  • Others:

    Author, as appears in the article.: Jordi Riu; M. Azizur R. Khana; Felismina T.C. Moreira; M. Goreti F. Sales
    Department: Química Analítica i Química Orgànica
    URV's Author/s: RIU RUSELL, JORDI; M. Azizur R. Khana; Felismina T.C. Moreira; M. Goreti F. Sales
    Keywords: Chemical detection Electropolymerization bacteria
    Abstract: This work presents a novel molecularly imprinted polymer (MIP) for the indirect detection of bacteria, by targeting an outer membrane protein on a disposable device. Protein A (PA) was selected for this purpose, as a representative protein of the outer surface of Staphylococcus aureus. The imprinted polymer was assembled directly on a film of single walled carbon nanotubes (SWCNTs), placed on screen-printed electrodes (SPEs). The MIP material was produced by electropolymerizing 3-aminophenol in the presence of the protein template (PA) using cyclic voltammetry (CV). The proteins entrapped at the polymeric backbone were digested by the action of proteolytic activity of proteinase K and then washed away to create vacant sites. The performance of the corresponding imprinted and non-imprinted electrodes was evaluated by EIS and the effect of several variables, such as monomer and template concentrations, or thickness of imprinting surface, was controlled and optimized by the number of CV cycles. The detection limit of the MIP-based sensors was 0.60 nM in MES buffer. High repeatability and good selectivity were observed in the presence of a model protein BSA. The sensor performance was also tested to check the effect of inorganic ions in tap water. The detection limit observed was 16.83 nM, with a recovery factor of 91.1 ± 6.6%. The sensor described in this work is a potential tool for screening PA on-site, due to the simplicity of fabrication, disposability, short response time, low cost, good sensitivity and selectivity.
    Research group: Grup de Quimiometria, Qualimetria i Nanosensors
    Thematic Areas: Chemistry Química Química
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 0925-4005
    Author identifier: 0000-0001-5823-9223; n/a; n/a; n/a
    Record's date: 2016-05-23
    Last page: 704
    Journal volume: 233
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0925400516305573?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.snb.2016.04.075
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    First page: 697
    Publication Type: Post-print
  • Keywords:

    Bacterial surface proteins
    Polimerització
    Bacteris
    Chemical detection
    Electropolymerization
    bacteria
    Chemistry
    Química
    Química
    0925-4005
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