Author, as appears in the article.: Lluis Arola; Josep M del Bas; Antoni Caimari; Maria Isabel Rodriguez-Naranjo; Caroline E Childs; Carolina Paras Chavez; Annette L West; Elizabeth A Miles; Philip C Calder
Department: Bioquímica i Biotecnologia
URV's Author/s: AROLA FERRER, LUIS MARIA; Josep M del Bas; Antoni Caimari; Maria Isabel Rodriguez-Naranjo; Caroline E Childs; Carolina Paras Chavez; Annette L West; Elizabeth A Miles; Philip C Calder
Keywords: fatty liver disease fish oil inflammation
Abstract: Plasma lysophospholipids have emerged as signaling molecules with important effects on inflammation, insulin resistance, and fatty liver disease, each of which is linked closely to obesity. Dietary n-3 (ω-3) polyunsaturated fatty acids (PUFAs) may be able to improve these conditions. Objective: The objective of this study was to assess the response of plasma lysophospholipids to obesity, n-3 PUFA consumption, and a high-fat meal challenge to better understand the role of lysophospholipid metabolism in the progression of obesity-related disorders. Design: We determined the concentrations of 8 lysophosphatidylcholines, 11 lysophosphatidylethanolamines, and 7 lysophosphatidylinositols in the plasma of 34 normal-weight and 38 obese subjects randomly assigned to consume corn oil (control) or n-3 PUFA-rich fish oil (3 g/d; n = 15-19/group) for 90 d. Blood samples were collected on the last day of the study under fasting conditions and 6 h after a high-fat meal (1135 kcal, 86 g fat) challenge. The profile of secreted lysophospholipids was studied in HepG2 cells under palmitate-induced steatosis. Results: Obese and normal-weight subjects had different profiles of plasma lysophospholipids. A multivariate combination of the 26 lysophospholipids could discriminate between normal-weight and obese subjects with an accuracy of 98%. The high-fat meal challenge altered the concentration of plasma lysophosphatidylcholines in an oil treatment-dependent manner in normal-weight but not obese subjects, suggesting that obesity impairs the sensitivity of lysophospholipid metabolism to n-3 PUFAs. Noncytotoxic steatosis in HepG2 cells affected the secretion pattern of lysophospholipids, partially resembling the changes observed in the plasma of obese subjects. Conclusions: Obesity has a substantial impact on lysophospholipid metabolism, altering the plasma lysophospholipid profile and abolishing its sensitivity to dietary n-3 PUFAs. These effects could contribute to the onset or progression of alterations associated with obesity, such as inflammation, insulin resistance, and fatty liver disease.
Research group: Grup de Recerca en Nutrigenòmica
Thematic Areas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0002-9165
Author identifier: 0000-0003-2767-1974; 0000-0002-0700-2004; n/a; 0000-0003-2180-0974; 0000-0001-6832-224X; n/a; 0000-0002-3331-0684; 0000-0002-8643-0655; n/a
Record's date: 2016-09-19
Last page: 279
Journal volume: 104
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: http://ajcn.nutrition.org/content/104/2/266
Article's DOI: 10.3945/ajcn.116.130872
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
First page: 266
Publication Type: Article Artículo Article
Repositori URV