Author, as appears in the article.: Perez-Laguna, Vanesa; Rezusta, Antonio; Jose Ramos, Juan; Miguel Ferrer, Luis; Gene, Josepa; Jose Revillo, Maria; Gilaberte, Yolanda;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: GENÉ DÍAZ, JOSEPA; Pérez-Laguna, V.; Rezusta, A.; Ramos, J.J.; Ferrer, L.M.; Revillo, M.J.; Gilaberte, Y.
Keywords: Photodynamic therapy
Abstract: Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.
Research group: Unitat de Micologia i Microbiologia Ambiental
Thematic Areas: Health sciences Ciencias de la salud Ciències de la salut
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0921-4488
Record's date: 2017-04-19
Last page: 101
Journal volume: 150
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0921448817300792?via%3Dihub
Article's DOI: 10.1016/j.smallrumres.2017.03.011
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
First page: 97
Publication Type: Article Artículo Article
Repositori URV