Articles producció científica> Bioquímica i Biotecnologia

Anti-inflammatory role of Leptin in glial cells through p38 MAPK pathway inhibition

  • Identification data

    Identifier: PC:2636
    Authors:
    Patraca I, Martínez N, Busquets O, Martí A, Pedrós I, Beas-Zarate C, Marin M, Ettcheto M, Sureda F, Auladell C, Camins A, Folch J.
    Abstract:
    Filiació URV: SI
  • Others:

    Author, as appears in the article.: Patraca I, Martínez N, Busquets O, Martí A, Pedrós I, Beas-Zarate C, Marin M, Ettcheto M, Sureda F, Auladell C, Camins A, Folch J.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: PATRACA FIERRO, JOSÉ IVÁN; MARTINEZ LOPEZ, NOHORA MILENA; Martí, A.; Pedrós, I.; Beas-Zarate, C.; Marin, M.; Ettcheto, M.; Sureda, F.; Auladell, C.; Camins, A.; FOLCH LOPEZ, JAUME
    Keywords: Leptin
    Abstract: Background In the present work, we studied the modulatory effect of Leptin (Lep) against pro-inflammatory cytokines, tumour necrosis factor-alpha (TNFα), interleukin 1-beta (IL1β) and interferon-gamma (IFNγ), in primary glial cell cultures. Methods Glial cultures were treated with pro-inflammatory cytokines (TNFα, 20 ng/ml; IL1β, 20 ng/ml; IFNγ 20 ng/ml). Cells were pre-treated with Lep 500 nM, 1 h prior to cytokine treatment. NO released from glial cells was determined using the Griess reaction. Cell viability was determined by the MTT method. Protein expression was determined by western blot. Results Pre-treatment with 500 nM Lep produced an inhibitory effect on inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production after glial cells exposure to pro-inflammatory cytokines. Anti-inflammatory effect can be related to a decrease in P38 MAP Kinase (MAPK) pathway activity. Treatment of glial cell cultures with Lep also reduced the intrinsic apoptotic pathway (cytochrome c release and caspase-3 activation). Conclusions We suggest that Lep would act as an anti-inflammatory factor in glial cells exposed to pro-inflammatory cytokines, exerting its function on p38 MAPK pathway and reducing NO production.
    Research group: Farmacobiologia Cel.lular
    Thematic Areas: Bioquímica i biotecnologia Bioquímica y tecnología Biochemistry and technology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1734-1140
    Record's date: 2017-03-14
    Last page: 418
    Journal volume: 69
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1734114016304273
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.pharep.2016.12.005
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    First page: 409
    Publication Type: Article Artículo Article
  • Keywords:

    Leptina
    Neuròglia
    Citoquines
    Leptin
    Bioquímica i biotecnologia
    Bioquímica y tecnología
    Biochemistry and technology
    1734-1140
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